agarose gel dna extraction kit, application of dna extraction, automated rna extraction, bacterial dna extraction kit, bacterial genomic dna isolation protocol, blood dna extraction kit, cell lysis dna extraction, dna and rna extraction, dna can be extracted from, dna cleanup protocol, dna extraction buffer protocol, dna extraction from cell culture protocol, dna extraction from cells, dna extraction from tissue, dna extraction from whole blood, dna extraction kit price, dna gel, dna isolation kit price, dna isolation procedure, dna purification protocol, dna rna extraction, dna rna isolation, dna rna purification, dna separation, extract labs, extraction cell, fungal dna extraction, gel purification, gene purification, genomic dna extraction, genomic dna extraction from cells, genomic dna extraction from mammalian cells, genomic dna isolation, genomic dna isolation from blood, genomic dna library, genomic dna precipitation, genomic dna preparation, genomic dna purification, invitrogen dna isolation kit, isolation of dna from a named plant, mercaptoethanol in dna extraction, pcr purification, plant cell dna extraction, plant dna extraction methods, plant dna isolation, plant genomic dna extraction, plasmid dna extraction methods, plasmid isolation protocol, plasmid miniprep, preparation of lysis buffer for dna extraction, purification kit, purified dna, soil dna extraction kit, spin column dna purification, tissue dna extraction kit, tissue extraction, ways to extract dna, yeast dna extraction, yeast genomic dna extraction protocol, yeast genomic dna isolation protocol
Extraction of DNA or RNA must be done first before we can study and manipulate nucleic acids. There are many ways to extract different types of DNA. Most extraction techniques involve breaking the cell and destroying all unwanted macromolecules. Cells can be broken using a detergent solution called lysis buffer which can disrupt nuclear and cell membranes. Unwanted proteins are broken down using an enzyme called proteases and unwanted RNA are broken down using ribonucleases. The test tube used in performing the process is centrifuged to separate the DNA from the unwanted cell debris. Alcohol is then added to precipitate the DNA and forms into a visible white gelatinous mass. DNA samples can be stored in ultra-freezers (–80°C ) for many years.