Genes for Normal Cell Division Identified in a Minimal Cell
- Cell division is the process by which a cell splits into two or more cells.
- In bacteria, cell division can be called binary fission which is the primary method for reproduction.
- James F. Pelletier of Massachusetts Institute of Technology and his team revealed the genetic requirement for cell division in a genomically minimal bacterial cell.
- Minimal cell is a cell-like structure containing the minimal and sufficient number of components to be defined as living thing.
- Minimal cell offers a platform to define genes that are involved in core physiological processes.
- Minimal cell includes genes that are important for population growth.
- However, the physiology of its single cells remained unidentified.
- The researchers investigated the striking morphological variation in a minimal cell.
- They presented an approach to identify cell propagation and determined genes that are associated in cell morphology.
- They observed the intrinsic dynamics of the cell that may have caused irregular structural formation.
- Using reverse genetic approach, normal-shaped minimal cells were generated from the abnormal-shaped minimal cells.
- The normal-shaped minimal cells contain 19 genes not retained in morphologically irregular minimal cells.
- From the 19 genes, 7 were identified that are required to restore the normal shape on minimal cell.
- The 7 genes include two cell division genes (ftsZ and sepF), hydrolase, and four genes that encode proteins associated with membrane.
- The function of the ftsZ protein is to recruit other cell division proteins to produce a new cell wall between the dividing cells.
- The sepF protein interacts with ftsZ and is required in the late step of cell division.
- The substrate for the hydrolase is unknown.
- The function of the proteins encoded by the 4 genes is unknown.
- This result highlights the polygenic nature of cell division and morphology in a genomically minimal cell.
Pelletier, J. F., Sun, L., Wise, K. S., Assad-Garcia, N., Karas, B. J., Deerinck, T. J., Ellisman, M. H., Mershin, A., Gershenfeld, N., Chuang, R. Y., Glass, J. I., & Strychalski, E. A. (2021). Genetic requirements for cell division in a genomically minimal cell. Cell, S0092-8674(21)00293-2. Advance online publication. https://doi.org/10.1016/j.cell.2021.03.008 https://www.springer.com/gp/book/9789048199433  https://www.uniprot.org/uniprot/Q6GHP9  https://pubmed.ncbi.nlm.nih.gov/16420366
Date Published: August 9, 2016 Publisher: Public Library of Science Author(s): Yasser Abdelrahman, Scot P. Ouellette, Robert J. Belland, John V. Cox, Christopher M. Sassetti. http://doi.org/10.1371/journal.ppat.1005822 Abstract: Bacterial cell division predominantly occurs by a highly conserved process, termed binary fission, that requires the bacterial homologue of tubulin, FtsZ. Other mechanisms of bacterial cell division that … Continue reading
Date Published: April 8, 2008 Publisher: Public Library of Science Author(s): Julia Hatzold, Barbara Conradt, Julie Ahringer Abstract: Asymmetric cell division and apoptosis (programmed cell death) are two fundamental processes that are important for the development and function of multicellular organisms. We have found that the processes of asymmetric cell division and apoptosis can be … Continue reading
Date Published: February 4, 2014 Publisher: Public Library of Science Author(s): Ivonne M. Sehring, Bo Dong, Elsa Denker, Punit Bhattachan, Wei Deng, Birthe T. Mathiesen, Di Jiang, Michael Levine Abstract: A cytokinesis-like contractile mechanism is co-opted in a different developmental scenario to achieve cell elongation instead of cell division in Ciona intestinalis. Partial Text: Individual … Continue reading
Research Article: Pseudomonas aeruginosa Transmigrates at Epithelial Cell-Cell Junctions, Exploiting Sites of Cell Division and Senescent Cell Extrusion
Date Published: January 4, 2016 Publisher: Public Library of Science Author(s): Guillaume Golovkine, Eric Faudry, Stéphanie Bouillot, Sylvie Elsen, Ina Attrée, Philippe Huber, Matthew R. Parsek. http://doi.org/10.1371/journal.ppat.1005377 Abstract: To achieve systemic infection, bacterial pathogens must overcome the critical and challenging step of transmigration across epithelial barriers. This is particularly true for opportunistic pathogens such as … Continue reading
Date Published: June 1, 2018 Publisher: Public Library of Science Author(s): Octavio Martínez, M. Humberto Reyes-Valdés, Charalampos Spilianakis. http://doi.org/10.1371/journal.pone.0198222 Abstract: Living cells are highly complex systems comprising a multitude of elements that are engaged in the many convoluted processes observed during the cell cycle. However, not all elements and processes are essential for cell survival … Continue reading
Date Published: February 6, 2015 Publisher: Public Library of Science Author(s): Claude Gérard, John J. Tyson, Damien Coudreuse, Béla Novák, Jeffrey J. Saucerman Abstract: In present-day eukaryotes, the cell division cycle is controlled by a complex network of interacting proteins, including members of the cyclin and cyclin-dependent protein kinase (Cdk) families, and the Anaphase Promoting … Continue reading