How Karyotypes Are Prepared?


Related Posts:

A karyotype is a display of condensed chromosomes arranged in pairs. Karyotyping can be used to screen for defective chromosomes or abnormal numbers of chromosomes associated with certain congenital disorders, such as Down syndrome.

Source: Urry, Lisa A.. Campbell Biology (p. 256). Pearson Education. Kindle Edition

How Karyotypes Are Prepared? (Campbell Biology)

In the picture above, karyotypes are prepared from isolated somatic cells, which are treated with a drug to stimulate mitosis and then grown in culture for several days. Cells arrested when the chromosomes are most highly condensed—at metaphase—are stained and then viewed with a microscope equipped with a digital camera. An image of the chromosomes is displayed on a computer monitor, and digital software is used to arrange them in pairs according to their appearance.

– What is the process by which photographs of chromosomes are taken in order to determine the chromosome complement of an individual, including the number of chromosomes and any abnormalities?

Source: Urry, Lisa A.. Campbell Biology (p. 256). Pearson Education. Kindle Edition

The karyotype in the above picture shows the chromosomes from a human male (as seen by the presence of the XY chromosome pair), colored to emphasize their chromosome banding patterns. The size of the chromosome, position of the centromere, and pattern of stained bands help identify specific chromosomes. Although difficult to discern in the karyotype, each metaphase chromosome consists of two closely attached sister chromatids.

– What is a nucleic acid stain used in cytogenetics and for the histopathological diagnosis of malaria and other parasites?


Urry, Lisa A.. Campbell Biology. Pearson Education. Kindle Edition.


Related Research

Research Article: Is the Karyotype of Neotropical Boid Snakes Really Conserved? Cytotaxonomy, Chromosomal Rearrangements and Karyotype Organization in the Boidae Family

Date Published: August 5, 2016 Publisher: Public Library of Science Author(s): Patrik F. Viana, Leila B. Ribeiro, George Myller Souza, Hipócrates de Menezes Chalkidis, Maria Claudia Gross, Eliana Feldberg, Kunbo Wang. Abstract: Boids are primitive snakes from a basal lineage that is widely distributed in Neotropical region. Many of these species are both morphologically … Continue reading

Research Article: Karyotype, Sex Determination, and Meiotic Chromosome Behavior in Two Pholcid (Araneomorphae, Pholcidae) Spiders: Implications for Karyotype Evolution

Date Published: September 9, 2011 Publisher: Public Library of Science Author(s): Adriana E. Golding, Leocadia V. Paliulis, Corrie S. Moreau. Abstract: There are 1,111 species of pholcid spiders, of which less than 2% have published karyotypes. Our aim in this study was to determine the karyotypes and sex determination mechanisms of two species of pholcids: … Continue reading

Research Article: The Ancestral Eutherian Karyotype Is Present in Xenarthra

Date Published: July 21, 2006 Publisher: Public Library of Science Author(s): Marta Svartman, Gary Stone, Roscoe Stanyon, Barbara Trask Abstract: Molecular studies have led recently to the proposal of a new super-ordinal arrangement of the 18 extant Eutherian orders. From the four proposed super-orders, Afrotheria and Xenarthra were considered the most basal. Chromosome-painting studies with … Continue reading