monoclonal antibodies | definition

antibodies produced in vitro that only bind to a single epitope


Parker, N., Schneegurt, M., Thi Tu, A.-H., Forster, B. M., & Lister, P. (n.d.). Microbiology. Houston, Texas: OpenStax. Access for free at:



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The Clinical Uses of Monoclonal Antibodies

OpenStax Microbiology. Since the most common methods for producing monoclonal antibodies use mouse cells, it is necessary to create humanized monoclonal antibodies for human clinical use. Mouse antibodies cannot be injected repeatedly into humans, because the immune system will recognize them as being foreign and will respond to them with neutralizing antibodies. This problem can be minimized … Continue reading

Producing Monoclonal Antibodies

OpenStax Microbiology. Some types of assays require better antibody specificity and affinity than can be obtained using a polyclonal antiserum. To attain this high specificity, all of the antibodies must bind with high affinity to a single epitope. This high specificity can be provided by monoclonal antibodies (mAbs).  Unlike polyclonal antibodies, which are produced in live … Continue reading

Research Article: A simplified workflow for monoclonal antibody sequencing

Date Published: June 24, 2019 Publisher: Public Library of Science Author(s): Lena Meyer, Tomás López, Rafaela Espinosa, Carlos F. Arias, Christopher Vollmers, Rebecca M. DuBois, Andrew C. Gill. Abstract. The diversity of antibody variable regions makes cDNA sequencing challenging, and conventional monoclonal antibody cDNA amplification requires the use of degenerate primers. Here, we describe … Continue reading

Research Article: A Monoclonal Antibody Toolkit for C. elegans

Date Published: April 13, 2010 Publisher: Public Library of Science Author(s): Gayla Hadwiger, Scott Dour, Swathi Arur, Paul Fox, Michael L. Nonet, Michael Hendricks. Abstract: Antibodies are critical tools in many avenues of biological research. Though antibodies can be produced in the research laboratory setting, most research labs working with vertebrates avail themselves of the … Continue reading

Research Article: High-Efficiency Screening of Monoclonal Antibodies for Membrane Protein Crystallography

Date Published: September 8, 2011 Publisher: Public Library of Science Author(s): Hyun-Ho Lim, Yiling Fang, Carole Williams, Vladimir N. Uversky. Abstract: Determination of crystal structures of membrane proteins is often limited by difficulties obtaining crystals diffracting to high resolution. Co-crystallization with Fab fragments of monoclonal antibodies has been reported to improve diffraction of membrane proteins … Continue reading

Research Article: Monitoring Monoclonal Antibody Delivery in Oncology: The Example of Bevacizumab

Date Published: August 12, 2013 Publisher: Public Library of Science Author(s): Guillaume Nugue, Marie Bidart, Marie Arlotto, Mireille Mousseau, François Berger, Laurent Pelletier, Rossella Rota. Abstract: Developing therapeutic monoclonal antibodies paves the way for new strategies in oncology using targeted therapy which should improve specificity. However, due to a lack of biomarkers, a personalized … Continue reading

Research Article: Preparation of Mouse Monoclonal Antibody for RB1CC1 and Its Clinical Application

Date Published: March 1, 2012 Publisher: Public Library of Science Author(s): Yusuke Hama, Tokuhiro Chano, Takuma Inui, Kyoichi Matsumoto, Hidetoshi Okabe, Joseph Alan Bauer. Abstract: RB1-inducible coiled-coil 1 (RB1CC1; also known as FIP200) plays important roles in several biological pathways such as cell proliferation and autophagy. Evaluation of RB1CC1 expression can provide useful clinical information … Continue reading

Research Article: Generation of Monoclonal Antibodies against Highly Conserved Antigens

Date Published: June 30, 2009 Publisher: Public Library of Science Author(s): Hongzhe Zhou, Yunbo Wang, Wei Wang, Junying Jia, Yuan Li, Qiyu Wang, Yanfang Wu, Jie Tang, Derya Unutmaz. Abstract: Therapeutic antibody development is one of the fastest growing areas of the pharmaceutical industry. Generating high-quality monoclonal antibodies against a given therapeutic target is very … Continue reading

Research Article: Anti-Gb3 Monoclonal Antibody Inhibits Angiogenesis and Tumor Development

Date Published: November 26, 2012 Publisher: Public Library of Science Author(s): Ariane Desselle, Tanguy Chaumette, Marie-Hélène Gaugler, Denis Cochonneau, Julien Fleurence, Nolwenn Dubois, Philippe Hulin, Jacques Aubry, Stéphane Birklé, François Paris, Christina Lynn Addison. Abstract: Inhibiting the growth of tumor vasculature represents one of the relevant strategies against tumor progression. Between all the different pro-angiogenic … Continue reading