real-time PCR | definition

a variant of PCR involving the use of fluorescence to allow for the monitoring of the increase in double-stranded template during a PCR reaction as it occurs, allowing for the quantitation of the original target sequence


Parker, N., Schneegurt, M., Thi Tu, A.-H., Forster, B. M., & Lister, P. (n.d.). Microbiology. Houston, Texas: OpenStax. Access for free at:



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