Research Article: Antigenic Fingerprinting of H5N1 Avian Influenza Using Convalescent Sera and Monoclonal Antibodies Reveals Potential Vaccine and Diagnostic Targets

Date Published: April 21, 2009

Publisher: Public Library of Science

Author(s): Surender Khurana, Amorsolo L. Suguitan, Yonaira Rivera, Cameron P. Simmons, Antonio Lanzavecchia, Federica Sallusto, Jody Manischewitz, Lisa R. King, Kanta Subbarao, Hana Golding, Malik Peiris

Abstract: Using whole-genome-fragment phage display libraries, Hana Golding and colleagues identify the viral epitopes recognized by serum antibodies in humans who have recovered from infection with H5N1 avian influenza.

Partial Text: The recent spread of highly pathogenic (HP) H5N1 avian influenza viruses (AIV) among poultry and transmission of these viruses to humans raised concerns of a potential influenza pandemic. In preparation for such an event, world-wide efforts are under way to test and stockpile preventive vaccines, antiviral drugs, and passive immune therapies [1], [2].

GFPDL are a powerful tool to decipher all the primary antigenic sites in influenza viruses following natural exposure or vaccination. Previously, this approach led to the development of HIV-SELECTEST for differential diagnosis of HIV infections in vaccine recipients [9], [20]. In the current study, we used H5N1 GFPDL to identify recognition sites of antibodies in convalescent sera obtained from five Vietnamese individuals with a history of H5N1 infection and two H5-specific neutralizing MAbs derived from two of these survivors. The study identified antibodies targeting the HA, NA, and the M2e among other proteins. Furthermore we provide evidence that the PB1-F2, a putative viral virulence factor, was recognized by antibodies in all five convalescent sera of H5N1-infected individuals (mean end-point titers of ≥1∶500).

Source:

http://doi.org/10.1371/journal.pmed.1000049

 

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