Date Published: April 21, 2009
Publisher: Public Library of Science
Author(s): Surender Khurana, Amorsolo L. Suguitan, Yonaira Rivera, Cameron P. Simmons, Antonio Lanzavecchia, Federica Sallusto, Jody Manischewitz, Lisa R. King, Kanta Subbarao, Hana Golding, Malik Peiris
Abstract: Using whole-genome-fragment phage display libraries, Hana Golding and colleagues identify the viral epitopes recognized by serum antibodies in humans who have recovered from infection with H5N1 avian influenza.
Partial Text: The recent spread of highly pathogenic (HP) H5N1 avian influenza viruses (AIV) among poultry and transmission of these viruses to humans raised concerns of a potential influenza pandemic. In preparation for such an event, world-wide efforts are under way to test and stockpile preventive vaccines, antiviral drugs, and passive immune therapies , .
GFPDL are a powerful tool to decipher all the primary antigenic sites in influenza viruses following natural exposure or vaccination. Previously, this approach led to the development of HIV-SELECTEST for differential diagnosis of HIV infections in vaccine recipients , . In the current study, we used H5N1 GFPDL to identify recognition sites of antibodies in convalescent sera obtained from five Vietnamese individuals with a history of H5N1 infection and two H5-specific neutralizing MAbs derived from two of these survivors. The study identified antibodies targeting the HA, NA, and the M2e among other proteins. Furthermore we provide evidence that the PB1-F2, a putative viral virulence factor, was recognized by antibodies in all five convalescent sera of H5N1-infected individuals (mean end-point titers of ≥1∶500).