Research Article: ARCHITECT HIV Combo Ag/Ab and RealTime HIV-1 Assays Detect Diverse HIV Strains in Clinical Specimens

Date Published: March 01, 2018

Publisher: Mary Ann Liebert, Inc.

Author(s): Mary A. Rodgers, Ana S. Vallari, Julie Yamaguchi, Vera Holzmayer, Barbara Harris, Coumba Toure-Kane, Souleymane Mboup, Samar Badreddine, Carole McArthur, Nicaise Ndembi, Dora Mbanya, Lazare Kaptue, Gavin Cloherty.


Periodic evaluation of the impact of viral diversity on diagnostic tests is critical to ensure current technologies are keeping pace with viral evolution. To determine whether HIV diversity impacts the ARCHITECT HIV Combo Ag/Ab (HIV Combo) or RealTime HIV-1 (RT) assays, a set of N = 199 HIV clinical specimens from Cameroon, Senegal, Saudi Arabia, and Thailand were sequenced and tested in both assays. The panel included historical groups N and P specimens and a newly identified group N specimen. These and specimens classified as H, U (unclassified)/URF (unique recombinant form), CRF (circulating recombinant form) 01, 02, 06, 09, 11, 13, 18, 22, 37, and 43 were detected by both the RT assay (1.75–6.84 log copies/ml) and the HIV Combo assay (3.26–1121.96 sample to cutoff ratios). Sequence alignment identified 3 or fewer mismatches to the RT assay oligos in 82.4% of samples. Altogether, these data demonstrate the HIV Combo and RT assays detect diverse strains of HIV in clinical specimens.

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The polymerase sequences have been deposited in Genbank under accession numbers MG012032-MG012216 and envelope sequences have been deposited under accession numbers MG022442-MG022621.




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