Date Published: September 8, 2011
Publisher: Hindawi Publishing Corporation
Author(s): Shuzo Urata, Juan Carlos de la Torre.
Several arenaviruses cause hemorrhagic fever disease in humans and pose a significant public health concern in their endemic regions. On the other hand, the prototypic arenavirus LCMV is a superb workhorse for the investigation of virus-host interactions and associated disease. The arenavirus small RING finger protein called Z has been shown to be the main driving force of virus budding. The budding activity of Z is mediated by late (L) domain motifs, PT/SAP, and PPXY, located at the C-terminus of Z. This paper will present the current knowledge on arenavirus budding including the diversity of L domain motifs used by different arenaviruses. We will also discuss how improved knowledge of arenavirus budding may facilitate the development of novel antiviral strategies to combat human pathogenic arenaviruses.
Arenaviruses are enveloped viruses with a bisegmented negative strand (NS) RNA genome with coding capability for four known genes: nucleoprotein (NP), surface glycoprotein precursor (GPC), polymerase (L), and matrix-like (Z) proteins. Despite their limited genome and proteomic complexity, arenaviruses are able to exhibit very different phenotypic infection outcomes ranging from long-term subclinical chronic infections on their natural rodent hosts  to hemorrhagic fever (HF) disease in humans, infected through mucosal exposure to aerosols or by direct contact of abrade skin with infectious material. Thus, Lassa virus (LASV), the causative agent of Lassa fever (LF) is estimated to infect several hundred thousand individuals yearly in its endemic regions of West Africa, resulting in a high number of LF cases associated with high morbidity and significant mortality. Likewise, Junin virus (JUNV) causes Argentine HF, a severe illness with hemorrhagic and neurological manifestations and a case fatality of 15–30%, whereas the Machupo (MACV) and Guanarito (GTOV) arenaviruses emerged as causative agents of HF in Bolivia and Venezuela, respectively. On the other hand, the prototypic arenavirus, lymphocytic choriomeningitis virus (LCMV), is a superb workhorse for the investigation of virus-host interactions including mechanisms of virus control and clearance by the host immune defenses, as well as viral counteracting measures leading to chronic infection and associated disease [2, 3]. Moreover, evidence indicates that the globally distributed prototypic arenavirus LCMV is a neglected human pathogen of clinical significance, especially in cases of congenital infection. In addition, LCMV poses a special threat to immunocompromised individuals, as illustrated by cases of transplant-associated infections by LCMV with a fatal outcome in the USA and Australia. Public health concerns about arenavirus infections are aggravated by the lack of licensed vaccines and current therapy being limited to the use of the nucleoside analog ribavirin, which is only partially effective, requires early and intravenous administration for optimal activity, and can cause significant side effects. Therefore, it is important to develop novel and effective antiarenaviral strategies, a task that should be facilitated by a better understanding of the arenavirus molecular and cell biology.
Arenaviruses are enveloped viruses with a bisegmented negative strand (NS) RNA genome and a life cycle restricted to the cell cytoplasm. Virions are pleomorphic but often spherical and covered with surface glycoprotein spikes. Both the large, L (ca 7.3 kb) and small, S (ca 3.5 kb) genome RNA species use an ambisense-coding strategy to direct the synthesis of two polypeptides in opposite orientation, separated by a noncoding intergenic region (IGR) with a predicted folding of a stable hairpin structure  (Figure 1). The S RNA encodes the viral glycoprotein precursor, GPC, (ca 75 kDa) and the nucleoprotein, NP, (ca 63 kDa), whereas the L RNA encodes the viral RNA-dependent RNA polymerase (RdRp, or L polymerase) (ca 200 kDa) and a small (ca 11 kDa) RING finger protein Z that is functionally the counterpart of the matrix (M) protein found in many enveloped NS RNA viruses.
Results derived from minigenome- (MG-) based assays identified NP and L as the minimal viral transacting factors required for efficient RNA synthesis mediated by the virus polymerase [10–12]. Z was not required for RNA replication or transcription, but rather Z has been shown to exhibit a dose-dependent inhibitory effect on both transcription and replication of LCMV, Tacaribe virus (TACV), and LASV MGs [10, 12–14]. The inhibitory activity of Z on RNA synthesis by the LCMV polymerase did not require the N-terminus or C-terminus of Z, whereas the RING domain was strictly required but not sufficient [13, 14]. RING domains are known to mediate protein-protein interactions, and Z protein has been documented to interact with a variety of host cellular proteins including PML [15, 16] and translation initiation factor eIF4E [15–18]. The Z-PML interaction was reported to result in disruption of PML nuclear bodies and redistribution of PML to the cytoplasm, but the biological implications of this remain to be determined. On the other hand the Z-eIF4E interaction was found to impair eIF4E-dependent translation through its RING domain . Interestingly, expression of Interferon regulatory factor 7 (IRF7), a key factor in the regulation of type I interferon (IFN) production by pDCs, is highly dependent on 4E . Therefore, it is plausible that Z might mediate inhibition of IRF7 expression in arenavirus-infected pDCs and, thus, contributing to the mechanisms by which arenaviruses overcome the innate immune response by the host.
The arenavirus Z protein has been shown to have bona fide budding activity [21–23]. Many enveloped viruses possess a matrix (M) protein that is often the main driving force of viral budding. Accordingly, the sole expression of this M protein can produce virus-like particles (VLPs). Frequently M proteins contain short amino acid motifs, called L (late) domains that play a critical role in virus budding. To date, the sequences PT/SAP, PPXY, and YPXnL (YPXL) have been well established as L domain motifs [24–26]. In addition to these L domains, the FPDL motif and several other short amino acid motifs have also been reported to function as L domains [24–26]. These L domain motifs exert their activity in virus budding by mediating the interaction with specific host cellular factors. Thus, the PT/SAP motif binds to Tsg101, a component of ESCRT-I (endosomal sorting complex required for transport-I) and initiates the budding process [27, 28]. Vps4A/B is AAA-type ATPases involved in catalyzing the disassembly and recycling of the membrane-bound ESCRT complexes [29–31]. Evidence indicates that the M protein of many, but not all, enveloped viruses have the ability to recruit ESCRT complex to their budding sites [24–26]. In addition to M, several other viral proteins, including Sendai virus (SeV) C protein and Marburg virus (MARV) NP, have been found to bind directly to ESCRT proteins, Alix/AIP1, or both and contribute to the budding process [24, 32–35]. Interestingly, some enveloped viruses, including influenza, are able to execute very efficiently the budding process without the ESCRT machinery [24, 36]. It is worth noting that although the M protein of VSV contains both PTAP and PPPY L domain motifs that interact with Tsg101 and Nedd4; respectively, Tsg101 and Vps4A were not required for efficient budding of VSV . Rous sarcoma virus (RSV) Gag has a PPPY motif whose activity has been shown to be regulated by late-domain-interacting protein (LDI-1, Nedd4 chicken homolog) [38, 39]. Recently LYPSL motif in RSV Gag was shown to serve as a second L domain motif . Table 1 summaries the variety of interactions observed between L domains present in M proteins of enveloped viruses and their host cellular interacting partners.
As with many other bona fide viral budding proteins, Z-mediated budding requires its interaction with specific cellular factors within the endosomal/multivesicular body pathway as we discuss below. The identification and characterization of LASV-Z-host protein interactions involved in virus budding may uncover novel anti-arenavirus targets, and facilitate the development of screening strategies to identify drugs capable of disrupting viral budding and thereby preventing virus propagation. The ability of Z to direct self-budding in the absence of other viral proteins should facilitate the development of assays amenable to both genetics and chemical High Throughput Screening (HTS) to identify host cellular proteins required for Z-mediated budding, as well as small molecule inhibitors of this process. To this end, the emergence of RNA interference as a pathway that allows the modulation of gene expression has enabled functional genetic screens in mammalian cell types. Likewise, combinatorial chemical libraries have emerged as a leading source of compounds for biological screens, and; therefore, it should be feasible to identify small molecule inhibitors of Z-mediated budding by screening chemical libraries using appropriately designed cell-based assays of Z-mediated budding. In this regard, recent findings have shown that the fusion of the smaller (185 amino acids) luciferase from Gaussia princeps (GLuc) to Z resulted in a chimeric protein (Z-GLuc) that retain wild-type Z budding activity that could be monitored by direct measuring of GLuc activity in tissue culture supernatant of Z-GLuc transfected cells. Initial studies have shown that this Z-GLuc-based budding assay consistently exhibits high signal-to-noise ratio (S/N) values (average 10-fold) , suggesting that it should be amenable for the development of both genetic and chemical HTS to identify host cellular genes contributing to Z-mediated budding and small molecule inhibitors of Z-mediated budding, respectively.
The ESCRT machinery was originally identified as the Class E subset of vacuolar protein sorting (VPS) genes required for the correct sorting of soluble hydrolases from the yeast Golgi to the vacuole [51–53]. The essential VPS-mediated sorting step occurs during MVB (multi vesicular body) formation, when ubiquitinated proteins and lipids present on the limiting endosomal membrane are recognized and sorted into endosomal membrane microdomains, which ultimately invaginate and form vesicles that bud into the lumen to create the MVB [54–56]. Vesicles of the MVB subsequently fuse with lysosome, thereby exposing the internal vesicles to the degrading lipases and proteases in this organelle. ESCRT pathway is also involved in the membrane abscission event at the conclusion of cell division [57, 58]. ESCRT-I contains Tsg101, Vps37, Vps23, and Mvb12A/B and has been shown to be recruited from the cytoplasm to the surface of maturing endosomes [29, 54–56, 59, 60]. Components of ESCRT-I, especially Tsg101, recognize ubiquitinated protein cargos and interact with ESCRT-II that participates in protein sorting and vesicle formation. It should be noted that Alix/AIP1 was found to bridge ESCRT-I and ESCRT-III using a different way to that used by ESCRT-II [61, 62]. ESCRT-III components also recruit another class E proteins, Vps4A/B, which are AAA-type ATPases involved in catalyzing the disassembly and recycling of the membrane-bound ESCRT complexes [29–31]. Notably, the abscission event during cellular cytokinesis, a process topologically similar to endosomal vesicle formation, utilizes the same ESCRT machinery [29, 58, 63].
Tetherin was identified as an IFN-inducible antiviral cellular factor that tether HIV virions at the PM [66, 67]. Subsequently studies have extended these findings to other viruses . As with other host innate immune defense factors, several viruses have evolved mechanisms to counteract tetherin-mediated antiviral activity . Tetherin has been shown to inhibit LASV Z-mediated budding . Accordingly, 293T cells constitutively expressing tetherin resulted in decreased production levels of LASV and MACV virion particle production, whereas siRNA-mediated knockdown of endogenous tetherin in HeLa cells resulted in increased production levels of LASV and MACV virion particle production. These results would suggest that LASV does not possess any tetherin-antagonizing function as described for other viral proteins including HIV-1 Vpu, EBOV GP, and KSHV K5 [66, 71–73].
Current evidence indicates that many enveloped viruses use the ESCRT machinery to exit from the cell. In the case of arenavirus budding, Z-Tsg101 interaction appears to facilitate access of Z to the ESCRT machinery. Despite significant recent progress in defining the basic aspects of arenavirus budding, there are still a large number of issues that have not been investigated including: (1) Identification and functional characterization of host cellular factors that interact with the PPXY L domain motif present in LASV, LCMV, and some other arenavirus Z to facilitate virus budding. PPXY L domain motifs present in EBOV and MARV VP40 have shown to mediate interaction with Nedd4.1 [74–76]. Likewise, for several retroviruses, the interaction of Gag PPXY L domains with ubiquitin ligases has been shown to contribute to the regulation of viral budding [24, 25, 38, 39]. How ubiquitin ligases may regulate budding remains unknown, but recent published data have shown arrestin-related proteins to connect ubiquitin ligases and the ESCRT machinery . It is important to know whether specific ubiquitin ligase may regulate Z-mediated budding, and if so, what are the mechanism underlying this regulation. (2) Ubiquitin or ubiquitin-like molecules (UBLs) have been shown to modify the properties and budding activity of HIV-1 Gag and EBOV VP40 proteins . It is currently unknown whether these protein modifiers may also have a role in the regulation of arenavirus budding. (3) The mechanisms by which arenavirus RNP interacts with Z and GP to form budding mature infectious progeny are largely unknown. (4) Whether the species-specific and type of cell influence arenavirus budding and the biological implications regarding the outcome of infection are issues that have not been investigated.