Research Article: Bacteriophage Isolated from Sewage Eliminates and Prevents the Establishment of Escherichia Coli Biofilm

Date Published: March 18, 2018

Publisher: Tabriz University of Medical Sciences

Author(s): Karla Veloso Gonçalves Ribeiro, Cleberson Ribeiro, Roberto Sousa Dias, Silvia Almeida Cardoso, Sergio Oliveira de Paula, Jose Cola Zanuncio, Leandro Licursi de Oliveira.


Purpose: Biofilm growth exerts a negative impact on industry and health, necessitating the development of strategies to control. The objective of this work was study the lytic activity of the phage isolated from the sewage network in the formation and degradation of Escherichia coli biofilms.

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Escherichia coli, one of the main components of the normal flora of the digestive tract of humans and animals,1 are usually harmless, although some strains are pathogenic and can cause intra- and extra-intestinal diseases.2 Specific gene groups and virulence factors facilitate the colonization, multiplication and survival of this bacterium in the host body.3

The increasing inefficiency of antibiotic therapy to eliminate biofilm-associated infections has accelerated the search for alternative therapeutic agents, including phages.22 Moreover, it has been shown that E. coli 30 can form more biofilm when grown in media with enrofloxacin, which is commonly used in the treatment of E. coli bovine mastitis, showing the importance to develop alternatives to the treatment of mastitis caused by this isolate.23

The authors would like to thanks the ‘Fundação de Amparo à Pesquisa do Estado de Minas Gerais – FAPEMIG’ and ‘Núcleo de Microscopia e Microanálise – NMM’ of the Universidade Federal de Viçosa.

Not applicable.

Authors declare no conflict of interest in this study




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