Research Article: Biolayer interferometry predicts ELISA performance of monoclonal antibody pairs for Plasmodium falciparum histidine-rich protein 2

Date Published: October 01, 2017

Publisher: Elsevier

Author(s): C.F. Markwalter, I.K. Jang, R.A. Burton, G.J. Domingo, D.W. Wright.


Predicting antibody pair performance in a sandwich format streamlines development of antibody-based diagnostics and laboratory research tools, such as enzyme-linked immunosorbent assays (ELISAs) and lateral flow immunoassays (LFAs). We have evaluated panels of monoclonal antibodies against the malarial parasite biomarker Plasmodium falciparum histidine rich protein 2 (HRP2), including 9 new monoclonal antibodies, using biolayer interferometry (BLI) and screened antibody pairs in a checkerboard ELISA. This study showed BLI predicts antibody pair ELISA performance for HRP2. Pairs that included capture antibodies with low off-rate constants and detection antibodies with high on-rate constants performed best in an ELISA format.

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PATH funded the development of the antibodies and entered into a licensing agreement with A&G to make the antibodies commercially available. PATH and its affiliated authors do not have any financial interest in A&G nor receive any royalties under the license agreement.




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