Research Article: Characteristics of Populations of the Russian Federation over the Panel of Fifteen Loci Used for DNA Identification and in Forensic Medical Examination

Date Published: , 2011

Publisher: A.I. Gordeyev

Author(s): V. A Stepanov, O.P. Balanovsky, A.V. Melnikov, A.Yu. Lash-Zavada, V.N. Khar’kov, T.V. Tyazhelova, V.L. Akhmetova, O.V. Zhukova, Yu.V. Shneider, I.N. Shil’nikova, S.A. Borinskaya, A.V. Marusin, M.G. Spiridonova, K.V. Simonova, I.Yu. Khitrinskaya, M.O. Radzhabov, A.G. Romanov, O.V. Shtygasheva, S.M. Koshel’, E.V. Balanovskaya, A.V. Rybakova, E.K. Khusnutdinova, V.P. Puzyrev, N.K. Yankovsky.



Seventeen population groups within the Russian Federation were characterized for the first time using a panel of 15 genetic markers that are used for DNA identification and in forensic medical examinations. The degree of polymorphism and population diversity of microsatellite loci within the Power Plex system (Promega) in Russian populations; the distribution of alleles and genotypes within the populations of six cities and 11 ethnic groups of the Russian Federation; the levels of intra- and interpopulation genetic differentiation of population; genetic relations between populations; and the identification and forensic medical characteristics of the system of markers under study were determined. Significant differences were revealed between the Russian populations and the U.S. reference base that was used recently in the forensic medical examination of the RF. A database of the allelic frequencies of 15 microsatellite loci that are used for DNA identification and forensic medical examination was created; the database has the potential of becoming the reference for performing forensic medical examinations in Russia. The spatial organization of genetic diversity over the panel of the STR markers that are used for DNA identification was revealed. It represents the general regularities of geographical clusterization of human populations over various types of genetic markers. The necessity to take into account a population’s genetic structure during forensic medical examinations and DNA identification of criminal suspects was substantiated.

Partial Text

Molecular genetic analysis methods are now widely applied in the identification of the biological samples of individuals: victims of crimes, disasters, and acts of terrorism, criminals, and contingent of special divisions of armies or law enforcement. A genetic DNA analysis in forensic medical examinations has two stages. At the first stage, the DNA characteristics of the samples collected at the locus delicti are determined. At the second stage, they are matched with the DNA collected from the suspects or relatives of the victims. If there is no match of the genotypes, that points to the fact that the samples examined do not belong to the individual in question (taking into account the exclusion probability). When genotypes match, the probability of their random matching, i.e., the probability that other individuals may have the same genotypes, is also taken into account.


Genetic variability of 15 STR PowerPlex 16

Estimates of the genetic variability of microsatellite loci, which are used for DNA identifications that comply with the international standards for such studies, were obtained for the first time in this study for the population of Russia and neighboring countries. The informativeness and resolution capacity of the full panel of STR loci was first estimated, and the reference allelic frequencies for Russian urban populations, certain ethnic groups of the Russian Federation, and neighboring countries were obtained.