Research Article: Derivation of Induced Pluripotent Stem Cells from Fetal Human Skin Fibroblasts

Date Published: July , 2010

Publisher: A.I. Gordeyev

Author(s): S.P. Medvedev, A.A. Malakhova, E.V. Grigor’eva, A.I. Shevchenko, E.V. Dementyeva, I.A. Sobolev, I.N. Lebedev, A.G. Shilov, I.F. Zhimulev, S.M. Zakian.



The isolation and study of autologous human stem cells remain among the most urgent problems
in cell biology and biomedicine to date. Induced pluripotent stem cells can be derived from
human somatic cells by the overexpression of a number of genes. In this study we reprogrammed
fetal human skin fibroblasts by transduction with retroviral vectors carrying murine
Oct4 , Sox2 , Klf4 , and c–Myc
cDNAs. As a result, cells with the protein expression and gene transcription pattern
characteristic of human embryonic stem cells were derived. These induced pluripotent cells are
capable of differentiation in vitro into the ectoderm, mesoderm, and
endoderm derivatives.

Partial Text

Induced pluripotent stem cells are a unique model for studies in many fields of biomedicine,
such as the molecular basis of human cell pluripotency and reprogramming (processes occurring
in early embryogenesis) [1]. Broad prospects are opening
up for the application of induced pluripotent stem cells in toxicology and pharmacology, as
well as in regenerative medicine [2–4].