Research Article: Determination and Quantification of the Vinblastine Content in Purple, Red, and White Catharanthus Roseus Leaves Using RP-HPLC Method

Date Published: March 18, 2018

Publisher: Tabriz University of Medical Sciences

Author(s): Rohanizah Abdul Rahim, Nor Hazwani Ahmad, Khaldun Mohammad Al Azzam, Ishak Mat.


Purpose: To determine and quantify vinblastine in different varieties of Catharanthus roseus using reversed-phase HPLC method.

Partial Text

Catharanthus roseus, commonly known as Madagascar periwinkle which belongs to the Apocynaceae family. It grows naturally as a wild flower throughout Africa, America, Australia, Asia, Southern Europe and on some islands in the Pacific Ocean. The Catharanthus roseus is widely studied and used as plant due to its pharmaceutical value that comes from its diversity of useful terpenoid indole alkaloids. The most valuable bisindole alkaloids are vincristine and vinblastine due to their antineoplastic activity in the treatment of many types of cancers.1

Experimental data were analyzed using t-test (SPSS Version 13.0, USA). Throughout the analysis, F-values (P < 0.05) were considered significant. This study proved the existence of vinblastine in Catharanthus roseus leaves using reversed-phase HPLC method. Purple variety has 1.2 times and 1.5 times more vinblastine concentrations as compared to the white variety and pink variety, respectively. A simple extraction procedure had effectively yielded vinblastine after separation in crude Catharanthus roseus aqueous extracts. This work has been supported by the Majlis Kanser Nasional (MAKNA). Not applicable All authors declare that she has no conflict of interest.   Source:


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