Research Article: Diauxic growth of Clostridium acetobutylicum ATCC 824 when grown on mixtures of glucose and cellobiose

Date Published: May 22, 2018

Publisher: Springer Berlin Heidelberg

Author(s): Felipe Buendia-Kandia, Emmanuel Rondags, Xavier Framboisier, Guillain Mauviel, Anthony Dufour, Emmanuel Guedon.


Clostridium acetobutylicum, a promising organism for biomass transformation, has the capacity to utilize a wide variety of carbon sources. During pre-treatments of (ligno) cellulose through thermic and/or enzymatic processes, complex mixtures of oligo saccharides with beta 1,4-glycosidic bonds can be produced. In this paper, the capability of C. acetobutylicum to ferment glucose and cellobiose, alone and in mixtures was studied. Kinetic studies indicated that a diauxic growth occurs when both glucose and cellobiose are present in the medium. In mixtures, d-glucose is the preferred substrate even if cells were pre grown with cellobiose as the substrate. After the complete consumption of glucose, the growth kinetics exhibits an adaptation time, of few hours, before to be able to use cellobiose. Because of this diauxic phenomenon, the nature of the carbon source deriving from a cellulose hydrolysis pre-treatment could strongly influence the kinetic performances of a fermentation process with C. acetobutylicum.

Partial Text

Lignocellulosic biomass represents an interesting alternative to fossil carbon resources (McKendry 2002; Mosier et al. 2005; Wyman et al. 2005; Briens et al. 2008; Wettstein et al. 2012; Nanda et al. 2014).

In this study, fermentations of glucose and/or cellobiose by C. acetobutylicum were performed in batch mode. Mono-substrate cultures showed that C. acetobutylicum is able to grow with cellobiose as efficiently as with glucose.




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