Research Article: Effects of Pediococcus parvulus 2.6 and its exopolysaccharide on plasma cholesterol levels and inflammatory markers in mice

Date Published: December 13, 2012

Publisher: Springer

Author(s): Cecilia Lindström, Olle Holst, Lars Nilsson, Rickard Öste, Kristina E Andersson.


Intake of dietary fibres may reduce the prevalence of physiological risk factors of the metabolic syndrome, such as high plasma lipid levels and low-grade inflammatory state. Dietary fibres are usually of plant origin however microbial exopolysaccharides (EPSs) have analogue structures that could potentially exert similar physiological effects. Pediococcus parvulus 2.6 (Pd 2.6) excretes a ropy EPS and has previously shown probiotic potential. The aim of this work was to evaluate physiological effects of Pd 2.6 and its EPS in vivo. The live Pd 2.6 (both the ropy and non-ropy isogenic variant) and its purified EPS were fed to hypercholesterolemic LDL-receptor deficient mice for 6 weeks to investigate their effects on cholesterol levels and the inflammatory tone of the animals. Both variants of Pd 2.6 survived passage through the mouse gut fulfilling an important criterion of probiotics. The ability to produce EPS was conferring an advantage to survival (faecal recovery of 3.7 (1.9-8.7) vs. 0.21 (0.14-0.34) *108 CFU, P < 0.001, median and 25th and 75th percentiles). The ropy Pd 2.6 decreased the levels of soluble vascular cell adhesion molecule-1 compared to the EPS alone (591 ± 14 vs. 646 ± 13 ng/ml, P < 0.05). An increase in liver weight in mice fed the purified EPS was observed, but with no change in liver lipids. No changes in blood lipids were detected in any group. Further the EPS induced growth of the caecal tissue and increased the amount of caecal content showing bulking properties like that of a dietary fibre.

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Central obesity, hypertension, dyslipidaemia and insulin resistance are all independent risk factors for cardiovascular disease and type 2 diabetes. Co-occurrence of these metabolic abnormalities is stated as the metabolic syndrome (Eckel et al. 2010) which is associated with elevated levels of pro-inflammatory cytokines and inflammatory markers indicating a chronic inflammatory state (Galisteo et al. 2008). Life style intervention, including dietary changes, is suggested as a primary therapy for the metabolic syndrome (Grundy et al. 2005) and dietary fibres, including β-glucans, have been identified as important substances in the management of the metabolic abnormalities within the syndrome (Delzenne and Cani 2005; Galisteo et al. 2008). Recently the composition of the intestinal microflora has been acknowledged as an important factor in regulating the body weight (Bäckhed et al. 2004; Cani and Delzenne 2009; Ley et al. 2006) and could be used in fighting central adiposity. The microbiota may be altered by prebiotics that selectively stimulate the growth and/or activity of intestinal bacteria that can be associated with improvement of the host health. Such prebiotics should resist gastric acid and hydrolysis by host enzymes to be able to reach the gut and there be selectively fermented by the microbiota (Gibson et al. 2004). Another approach for modulation of physiological parameters is through the application of probiotics; living microorganisms which confer a health benefit to the host when administered in sufficient amounts (FAO/WHO 2002). The genus Bifidobacterium and species belonging to the group of lactic acid bacteria (LAB) like Lactobacillus are the most commonly used probiotics (Saad et al. 2013).

Dietary fibres, specifically β-glucans, have been recognized as important substances with potential to regulate certain physiological parameters like the glycaemic response, hypertension and dyslipidaemia. There are several reports on the beneficial effects of cereal (Tiwari and Cummins 2011) and fungal (Kim et al. 2005; Mattila et al. 2000) β-glucans. Information about the effect of β-glucans from LAB is however scarce. Studies of specific physiological effects for structurally different β-glucans in animal and clinical trials are of great interest and it is of importance to characterize the β-glucans used (Chen and Raymond 2008). Therefore in the present study a β-glucan of known structure, the 2-substituted (1,3)-β-D-glucan produced by Pd 2.6 (Duenas-Chasco et al. 1997), was investigated concerning its effects on plasma lipids and inflammatory markers. The EPS was containing 10% protein of unknown identity however the protein content was not taken into account when the EPS was added to the diet. The molar mass distribution of the EPS was determined using AF4-MALS-RI (Figure 1) and the main population (highest peak) was showing a molar mass distribution of about 106 Da in accordance with earlier reports (Lambo-Fodje et al. 2007). The population showing a higher molar mass (Figure 1) was of unknown origin however it could be an aggregated form of the EPS due to the high molar mass species present (M > 108 g/mol). Figure 1 also shows the strength of AF4 being able to separate species of a very wide size range. The EPS was added to the experimental diet solubilised in its native form. The yield of the EPS is not feasible for commercial production of pure EPS therefore in situ production would probably be a better option. It has been shown that the presence of a food matrix like orange juice may increase the survival of Pd 2.6 when exposed to gastric stresses. The food is not negatively affected by the presence of Pd 2.6 which means that it can be used as a mean to administer the EPS and bacteria in vivo easily (Elizaquivel et al. 2011).

RÖ has economical interest in Aventure AB that participated in the financing of the Antidiabetic Food Centre, which funded the study. CL, OH, LN and KEA declare that they have no competing interest.




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