Research Article: Effects of Pomegranate (Punica Granatum L.) Seed and Peel Methanolic Extracts on Oxidative Stress and Lipid Profile Changes Induced by Methotrexate in Rats

Date Published: June 30, 2017

Publisher: Tabriz University of Medical Sciences

Author(s): Farideh Doostan, Roxana Vafafar, Parvin Zakeri-Milani, Aliasghar Pouri, Rogayeh Amini Afshar, Mehran Mesgari Abbasi.


Purpose: Methotrexate (MTX) is prescribed in many diseases and can result in oxidative stress (OS) followed by injuries in some tissues. Antioxidants administration are effective in reducing OS. Pomegranate exhibits high anti-oxidant capacities. This study investigated whether pomegranate seed and peel methanolic extracts (PSE and PPE) could protect against MTX-induced OS and lipid profile changes in rats.

Partial Text

The presence of active oxygen species in excess of the tissue’s available antioxidant buffering capacity, results in oxidative stress. Reactive oxygen species (ROS) may damage DNA, proteins, lipids, and/or carbohydrates disturbing the cells or tissues structure and function. Tissue damage and sometimes chronic human diseases may occur following enzyme and non-enzyme-mediated biochemical reactions, which produce free radicals that are extremely reactive intermediate compounds. All body tissues are exposed chronically to oxidants from endogenous and/or exogenous sources.1,2

The results of this study showed the protective effects of PSE and PPE against MTX-induced serum oxidative stress (SOD and GPx) and lipid profile (TC, HDL, and LDL) changes in rats. The findings of this study also showed considerable antioxidant activity, total phenolic, and total flavonoid contents of PSE and PPE. However, further studies are needed to investigate the mechanisms of oxidative stress induction and protection, some un-expected results, and the controversies associated with previous studies.

The authors appreciate the members of the Drug Applied Research Center and Student Research Committee of Tabriz University of Medical Sciences (Tabriz, Iran) for their instrumental and financial support.

The animal experiments were ethically approved by Tabriz University of Medical Sciences Research Ethics Committee (code:5-4-110-60). All procedures were according to the Helsinki’s humanity research declaration.

The authors declare no conflict of interests.




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