Date Published: December 13, 2019
Publisher: Sociedade Brasileira para o Desenvolvimento da Pesquisa em
Author(s): Clarice Fraga Esteves Maciel Osorio, Waldemar Silva Costa, Carla Braga Mano Gallo, Francisco José Barcellos Sampaio.
To quantify and compare the expression of stromal elements in prostate
adenocarcinoma of different Gleason scores with non-tumor area
We obtained 132 specimens from samples of prostate peripheral and transition
zone. We analyzed the following elements of the extracellular matrix:
collagen fibers, elastic system, smooth muscle fibers and blood vessels. The
tumor area and non-tumor area (control) of the TMA (tissue microarray) were
photographed and analyzed using the ImageJ software.
The comparison between the tumor area and the non-tumor area showed
significant differences between stromal prostate elements. There was an
increase of collagen fibers in the tumor area, mainly in Gleason 7. Elastic
system fibers showed similar result, also from the Gleason 7. Blood vessels
showed a significant increase occurred in all analyzed groups. The muscle
fibers exhibited a different behavior, with a decrease in relation to the
There is a significant difference between the extracellular matrix in
prostate cancer compared to the non-tumor area (control) especially in
Gleason 7. Important modifications of the prostatic stromal elements
strongly correlate with different Gleason scores and can contribute to
predict the pathological staging of prostate cancer.
During the past decade, various methods have been used to determine the prognosis of
patients with prostate cancer (PC). Among them, is the histopathological
classification proposed by Gleason1.
This project was approved by the Ethics Committee (CAAE number 12685413.6.0000.5259)
– Universidade do Estado do Rio de Janeiro, Brazil.
Results are presented on Table 1, and Figures 1 and 2.
In a previous article, one of the authors of the present study showed a correlation
between the primary Gleason score and nuclear medium volume17. However, PC is not only a disease of abnormal epithelial cell
proliferation, but a disease that affects the complex interactions between prostatic
epithelial cells and stromal compartment18. Histopathological differences observed in varying Gleason scores, showed
that there is a constant change in the stromal of PC. The development of aggressive
neoplasm appears to be associated with the biosynthesis of the ECM and, therefore,
with changes in its structure15,19. Neoplastic epithelial cells, in interaction with stromal cells, and other
elements of the ECM, create a microenvironment susceptible to proliferation and
differentiation in carcinogenesis8.
Our data show that significant modifications of the prostatic stromal elements
strongly correlate with different Gleason scores, especially for the Gleason 7 or
higher, and can contribute to predicting pathological staging of prostate cancer.
Also, these data can contribute for the studies of the morphological substrate of