Date Published: January 27, 2010
Publisher: Public Library of Science
Author(s): Huaiyu Yang, Martin Bogner, York-Dieter Stierhof, Uwe Ludewig, Karin Schumacher. http://doi.org/10.1371/journal.pone.0008917
Abstract: Glutamine is one of the primary amino acids in nitrogen assimilation and often the most abundant amino acid in plant roots. To monitor this important metabolite, a novel genetically encoded fluorescent FRET-reporter was constructed and expressed in Arabidopsis thaliana. As a candidate for the glutamine fluxes, the root tip localized, putative amino acid transporter CAT8 was analyzed and heterologously expressed in yeast and oocytes.
Partial Text: Amino acid uptake from the soil by plant roots can play a significant role in ecosystems with low mineralization rates . While plants generally have the capacity to take up many amino acids, these often negatively interfere with biomass production and even inhibit growth . The model plant Arabidopsis has the capacity to acquire and utilize a number of amino acids for growth, including the amide glutamine (Gln) .