Research Article: H+-Independent Glutamine Transport in Plant Root Tips

Date Published: January 27, 2010

Publisher: Public Library of Science

Author(s): Huaiyu Yang, Martin Bogner, York-Dieter Stierhof, Uwe Ludewig, Karin Schumacher.

Abstract: Glutamine is one of the primary amino acids in nitrogen assimilation and often the most abundant amino acid in plant roots. To monitor this important metabolite, a novel genetically encoded fluorescent FRET-reporter was constructed and expressed in Arabidopsis thaliana. As a candidate for the glutamine fluxes, the root tip localized, putative amino acid transporter CAT8 was analyzed and heterologously expressed in yeast and oocytes.

Partial Text: Amino acid uptake from the soil by plant roots can play a significant role in ecosystems with low mineralization rates [1]. While plants generally have the capacity to take up many amino acids, these often negatively interfere with biomass production and even inhibit growth [2]. The model plant Arabidopsis has the capacity to acquire and utilize a number of amino acids for growth, including the amide glutamine (Gln) [3].



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