Research Article: Hepathic, biochemical, hematological, and histological effects of the ultracavitation in rabbits livers 1

Date Published: June 19, 2020

Publisher: Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia

Author(s): Patrícia Froes Meyer, Janiele Ferreira da Silva Sousa, Rejane Vilar da Rocha, José Queiroz, Oscar Ariel Ronzio, Rodrigo Marcel Valentim da Silva, Ana Camila de Medeiros Manso, Afra Rafaelli Magalhães de Almeida, Camila Procopio Andrada.


To collect data capable of pointing out the effects of the ultracavitation treatment on the liver of rabbits after adipose tissue application, by means of histological analyses of the liver and hematological and biochemical exams.

This is an experimental study with 12 albino rabbits as sample, which were divided into 3 groups and submitted to a hypercaloric diet for one month. Subsequently, subjects underwent UCV treatment: 3 minutes, 30 W, continuous mode at 100%, every 2 ERAS = 441.02 J/cm2, intensity of 10w/cm2. They were then euthanized and underwent biopsy after 24 hours.

After 48 hours from the ultracavitation treatment, the animals’ livers presented greater amount of fat infiltration if compared to the amount presented 96 hours after the treatment. However, laboratory tests showed no alterations. Values were maintained within normal parameters of cholesterol, triglycerides, liver enzymes, hemoglobin and hematocrit levels.

This study has identified that infiltrates may appear on livers after the treatment, despite high hematological and biochemical tests results. The fat infiltrates reduction 96 h after treatment suggests lower risks to animal health, if the period between applications is respected.

Partial Text

The high-intensity ultrasound device, also named ultracavitator , stands out among the non-invasive techniques that aim to safely and effectively meet the growing demand for methods of localized lipodystrophy reduction, even replacing previously utilized aggressive and invasive methods of adipose tissue reduction. This equipment is used in procedures of therapeutic purpose due to its efficient reduction of the adipose panicle, being recently reformulated and introduced in the segment of esthetic medicine1 , 2 .

It is an experimental study which was carried out in the laboratory of animal experimentation, in the Universidade Potiguar (UnP) laboratory.

The histological analysis showed increasing amount of fat infiltrates compared to Figure 2B (group G2). However, G1 presented less fat infiltrates.

A possible explanation for the results of the histological analysis to demonstrate the presence of fat infiltrates in the animals’ livers is that they were probably a consequence of the weight gain promoted by the hypercaloric diet the subjects underwent until treatment. However, in a comparative analysis, the treated groups (G1 and G3) presented greater amounts of fat infiltrates in the hepatic tissue. Such findings may indicate that the high intensity and high frequency protocol of UCV applied in this study was able to promote the increase of fat presence in the hepatic parenchyma, being its highest incidence 48 h after treatment, which reinforces the affirmations of a few studies1 – 4 . Despite being caged and not performing any physical activity, in 96 h after treatment, reduction in the amount of fat was identified.

The effects of the UCV treatment on rabbits’ livers showed an increase in fat infiltrates in the hepatic tissue 48h after treatment, evidenced in the quantitative analysis and in the histological descriptive analysis. However, there were no evident changes in the laboratory tests results, which leads to the conclusion that infiltrates may appear after treatment without the increase in the hematological and biochemical exams. A decrease in the quantity of fat infiltrates was observed 96h after treatment, suggesting lower risk to animal health if this range of applications is respected.




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