Research Article: High-throughput 16S rRNA gene sequencing reveals that 6-hydroxydopamine affects gut microbial environment

Date Published: August 12, 2019

Publisher: Public Library of Science

Author(s): Jin Gyu Choi, Eugene Huh, Namkwon Kim, Dong-Hyun Kim, Myung Sook Oh, David I. Finkelstein.


Recently, there has been a rapid increase in studies on the relationship between brain diseases and gut microbiota, and clinical evidence on gut microbial changes in Parkinson’s disease (PD) has accumulated. 6-Hydroxydopamine (6-OHDA) is a widely used neurotoxin that leads to PD pathogenesis, but whether 6-OHDA affects gut microbial environment has not been investigated. Here we performed the 16S rRNA gene sequencing to analyze the gut microbial community of mice. We found that there were no significant changes in species richness and its diversity in the 6-OHDA-lesioned mice. The relative abundance of Lactobacillus gasseri and L. reuteri probiotic species in feces of 6-OHDA-lesioned mice was significantly decreased compared with those of sham-operated mice, while the commensal bacterium Bacteroides acidifaciens in 6-OHDA-treated mice was remarkably higher than sham-operated mice. These results provide a baseline for understanding the microbial communities of 6-OHDA-induced PD model to investigate the role of gut microbiota in the pathogenesis of PD.

Partial Text

Parkinson’s disease (PD) is a multicentric neurodegenerative disease clinically defined by motor deficits and progressive degeneration of dopaminergic neurons in brain [1]. Non-motor manifestations, which precede the motor disabilities in PD patients, play a key role in the disease progression and evidence for their significance has gradually accumulated [2–4]. Among the non-motor symptoms of PD, gastrointestinal (GI) dysfunction, including drooling, impaired gastric emptying, and constipation are frequently reported [5, 6].

The composition of gut microbiota can have a remarkable impact on disease status as well as normal physiology in brain [31, 32]. Emerging evidence supports that gut dysbiosis by altered gut microbiota has the potential to be closely linked with neurodegenerative diseases, including PD [33–36]. In this study, we found that 6-OHDA, a well-known neurotoxin that leads to PD pathogenesis, creates gut microbial environment that affects specific microbes in mice using the high-throughput 16S rRNA gene sequencing method.




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