Research Article: Improvement of shelf life of soymilk using immobilized protease of Oerskovia xanthineolytica NCIM 2839

Date Published: August 8, 2016

Publisher: Springer Berlin Heidelberg

Author(s): A. K. Sahoo, V. S. Gaikwad, R. C. Ranveer, P. B. Dandge, S. R. Waghmare.


Protease enzyme has lot of commercial applications, so the cost-effective production of protease using sunflower oil seed waste was carried out from Oerskovia xanthineolyitca NCIM 2839. The maximum protease production was after 24 h of incubation with 2.5 % oil seed waste concentration. O. xanthineolytica was found to produce two proteases—P1 and P2. The proteases were purified using 60 % cold acetone precipitation and DEAE-cellulose ion exchange chromatography. SDS-PAGE revealed molecular weight of P1 and P2 was 36 and 24 kDa, respectively. P1 and P2 were optimally active at pH 7.0 and pH 7.5 at temperature 35 and 40 °C, respectively. Analysis of hydrolyzed product of P1 and P2 by HPLC reveals that the P1 has endoprotease and P2 has exoprotease activity. The treated soy milk with immobilized proteases showed increased shelf life and removal of off flavor.

Partial Text

Proteolytic enzymes constitute one of the most important groups of commercial enzymes. These enzymes have ample utilization in industrial processes, such as the detergent industry, a major consumer of proteases, as well as food and leather industries (Kumar and Takagi 1999; Gupta et al. 2002). Proteases are ubiquitous enzymes occurring in wide diversity of species including plants, animals and microorganisms. The vast range led to numerous attempts to exploit their biotechnological applications and established proteases as one of the major groups of industrial enzymes (Rao et al. 1998). Proteases are involved in numerous biological functions, such as septum formation, sporulation, protein turnover, catabolite inactivation, protein secretion and nutrition (Van Tilburg et al. 1984; Godfrey and West 1996).

In the present study, the low-cost production of protease from O. xanthineolytica NCIM 2839 using sunflower oil seed waste was carried out. As far as we are concerned, our work is the first contribution toward the production of protease using sunflower oil seed waste from O. xanthineolytica NCIM 2839. After the treatment of soy milk with protease, increased shelf life of soy milk encourages possible application of protease for the food industries.




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