Date Published: March 18, 2018
Publisher: Tabriz University of Medical Sciences
Author(s): Fatemeh Madani, Seyedeh Sara Esnaashari, Basil Mujokoro, Farid Dorkoosh, Masood Khosravani, Mahdi Adabi.
Purpose: The size of polymeric nanoparticles is considered as an effective factor in cancer therapy due to enterance into tumor tissue via the EPR effect. The purpose of this work was to investigate the effective parameters on poly(lactic-co-glycolic acid)-paclitaxel (PLGA –PTX) nanoparticles size.
Cancer is a major global cause of morbidity and mortality which is estimated that the incidence of all new cancer cases will reach 22 million by 2030 in worldwide.1 Chemotherapy is a versatile cancer treatment modality due to its application as first line,2,3 adjuvant4 and/or palliative therapy5 in the fight against different cancers. In addition, chemotherapy is easier to administer and less invasive compared to other clinical cancer treatment modalities such as surgical removal and radiotherapy. Unfortunately, since the efficacy of most chemotherapeutic drugs is dose dependent, severe chemo-induced side events have been observed at higher doses.6-8 Thus targeted delivery of drugs with minimum non-specific exposure is essential for successful chemotherapy. Tumor targeting chemotherapy can be accomplished by exploiting the diseases’ pathophysiology such as unique or overexpressed molecules9 and leaky tumor vasculature.10
Paclitaxel was purchased from sigma. PLGA (50:50, MW 30000 g mol−1) was bought from Shenzhen Esun Industrial Co., China. Dichloromethane (DCM) and acetone (99%) supplied by Carol Erba. Polyvinyl alcohol (PVA), fully hydrolized (MW 60000 g mol−1) was obtained from Merck (Germany). All solutions were prepared using deionized water.
This study compared two methods for preparation of paclitaxel loaded PLGA nanoparticles including single emulsion and precipitation. The results indicated that precipitation method results in smaller nanoparticles compared to emulsion one. In addition, the effect of the various parameters on the size of the nanoparticles was investigated The results demonstrated that the concentration of the PLGA polymer, drug and PVA had a direct relationship with the size of nanoparticles whereas sonication time and organic phase to aqueous volume ratio needed to the optimization to obtain the nanoparticles with smaller sizes.
This work was supported by Tehran University of Medical Sciences, Grant No. 96-01-87-34138.
The authors declare no conflict of interests.