Date Published: March 7, 2018
Author(s): Chiara Nicolazzo, Luciano Colangelo, Alessandro Corsi, Guido Carpino, Angela Gradilone, Chiara Sonato, Cristina Raimondi, Eugenio Gaudio, Paola Gazzaniga, Walter Gianni.
Hemangiopericytoma (HPT) is a rare mesenchymal tumor of fibroblastic type and for its rarity is poorly studied. The most common sites of metastatic disease in patients with intracranial HPT are the bone, liver, and lung, suggestive for an hematogenous dissemination; for this reason, we investigated, for the first time, the presence of circulating tumor cells (CTCs) in hemangiopericytoma patient by CellSearch® and SceenCell® devices. Peripheral blood samples were drawn and processed by CellSearch, an EpCAM-dependent device, and ScreenCell®, a device size based. We found nontypical CTCs by CellSearch system and the immunofluorescence analysis performed on CTCs isolate by ScreenCell demonstrated the presence of single CTCs and CTC clusters. The molecular characterization of single CTCs and CTC clusters, using antibodies directed against EpCAM, CD34, cytokeratins (8, 18, and 19), and CD45, showed a great heterogeneity in CTC clusters. We believe that the present study may open a new scenario in the rare tumors: the introduction of the liquid biopsy and the molecular characterization of circulating tumor cells could lead to personalized targeted treatments and also for rare tumors.
Hemangiopericytoma (HPT) is a rare mesenchymal tumor of fibroblastic type . Although originally presumed to originate from vascular pericytes , the precise histogenesis has not been definitely established. From a mere clinical point of view, HPT represents an unpredictable disease, since distant relapses are usually delayed and may occur even decades after diagnosis of the primary tumors , suggesting that a long-term follow-up is strongly needed . Treatments are limited so far also due to inadequate funding for preclinical and clinical research programmes, and new strategies are mandatory in order to allow the earlier detection of tumor recurrence and metastasis, thus improving the diagnosis, treatment, and surveillance of patients. The most common sites of metastatic disease in patients with intracranial HPT are the bone, liver, and lung, suggestive for an hematogenous dissemination . Circulating tumor cells (CTCs) represent a unique source of information about hematogenous spread of tumors and are recognised as a useful tool in the identification of new clinically relevant biomarkers . Although CTCs have been mainly isolated from carcinomas through epithelial antigen-targeted antibodies (EpCAM), reports have demonstrated that EpCAM expression is not restricted to epithelial-derived tumors, but shared as well by CTCs isolated from patients with mesenchymal-derived cancers . In the present study, we sought to investigate the feasibility to isolate CTCs from a patient with secondary liver hemangiopericytoma using CellSearch system, the only FDA-approved for CTCs enumeration. CellSearch captures cells using a monoclonal antibody specific to EpCAM and identifies CTCs using differential fluorescent antibodies to detect the presence of cytokeratins (CK) within a nucleus-containing intact cell and absence of CD45, as defining characteristics of CTCs (DAPI+/CK+/EpCAM+/CD-45-).
According to the mesenchymal nature of hemangiopericytoma, we failed to detect typical epithelial-like CTCs, but we were able to identify 5 DAPI+/EpCAM+/CK-/CD45- events, which might be referable to cells with nonepithelial features. According to the CellSearch guidelines, all objects with no clear cytokeratin staining are defined as “suspicious objects.” Similar events have been previously described by our group to have prognostic significance in other cancer types, such as renal cell carcinoma, frequently lacking cytokeratin expression . In order to better characterize these atypical CTCs, we processed a second blood sample by using ScreenCell, a device that allows a size-selective separation of CTCs from other blood cells. To evaluate the molecular profile of atypical CTCs, an immunofluorescence staining was performed on two filters obtained from the blood of the patient, using in the first one the same antibodies used in the CellSearch system (EpCAM, CK, and CD-45, as defining characteristics of CTCs) and in the second one CD34 (as hemangiopericytoma specific marker) (Figure 1) [10, 11]. In both the examined filters, single or clustered CTCs were identified and an average of one cluster per filter was observed. CTCs were variably round or elongated, presented a high nucleus to cytoplasm ratio, scant cytoplasm, and an oval nuclei. In examined filters, both single and clustered CTCs were found positive for EpCAM but negative for CD45. EpCAM expression was localized at the cell surface in single cells, but retained in the internal part of the clusters. The absence of EpCAM at the cluster surface might explain the failure of CellSearch to detect CTC clusters, as already demonstrated in epithelial-like tumors, such as nonsmall cell lung cancer . EpCAM-positive clusters variably expressed CK but were constantly negative for CD45 and positive for CD34. Some studies have demonstrated that CTC clusters are composed of a number of tumor cells mixed to nontumor cells such as mesenchymal cells, epithelial cells, pericytes, immune cells, platelets, and cancer-associated fibroblasts . In our patient, the coexpression of EpCAM, CK, and CD34 within the CTC cluster confirms the great heterogeneity in CTC clusters and their patent multifaceted composition. The feasibility of a liquid biopsy in a rare tumor, such as HPC, might have several crucial clinical implications. First, most intracranial meningeal HPCs resemble meningiomas in their clinical presentation and histological features and may therefore be misdiagnosed, despite important differences in prognosis . Keeping scanning and following up with a noninvasive tool, such as a blood draw, might make the difference. Second, systemic therapy options for HPC are limited so far, and due to the increasing number of targeted therapies available, the molecular characterization of CTCs has the unique potential to translate into personalized treatments . To this purpose, clinical trials on rare cancers are not usually performed due to the small numbers of eligible patients, and while more common cancers are increasingly being treated according to a biomarker-driven approach, the “one size fits all” approach is still the standard of care for rare tumors. Personalized targeted treatments need to be developed specifically for hemangiopericytoma as well as in all other rare cancers, and liquid biopsy has certainly the right credentials to facilitate this process.