Research Article: Mesenchymal stem cells rejuvenate cardiac muscle after ischemic injury

Date Published: January 15, 2019

Publisher: Impact Journals

Author(s): Busheng Zhang, Jing Zhang, Dan Zhu, Ye Kong.


Previous studies have shown that transplantation of mesenchymal stem cells (MSCs) enhances myocardial regeneration after myocardial infarction (MI), primarily resulting from the production and release of trophic growth factors and cytokines by MSCs. However, effects of MSCs or a subtype of MSCs on the ageing of injured cardiac muscle cells (CMCs) are limitedly known. Here, we addressed this question. CD146+ MSCs were isolated from total MSCs (tMSCs), and their effects on injured CMCs were assessed. In vivo, transplantation of isogenic CD146+ MSCs into MI-mice increased the proliferation of CMCs and reduced apoptosis of CMCs in a significantly higher degree than transplantation of tMSCs, resulting in significant improvement of the heart function. In vitro, CMCs were co-cultured under hypoxia condition with CD146+MSCs or tMSCs. We found that CD146+MSCs increased the proliferation of CMCs and reduced apoptosis of CMCs in a significantly higher degree, compared to tMSCs, likely resulting from reduction of aging-associated cellular reactive oxygen species in CMCs. Together, these data suggest that MSCs rejuvenate CMCs after ischemic injury and a subtype of MSCs, CD146+ MSCs, appears to have higher potential in coordinating this process.

Partial Text

Coronary heart disease (CHD) is a prevalent disease with high morbidity. Prolonged impairment of physiological supply from coronary blood vessels results in ischemic cardiomyopathy and even myocardial infarction (MI), which causes heart failure [1]. Prevention from the dysfunction and loss of myocardial cells, and augmentation of the regeneration of cardiomyocytes after MI are both critical approaches leading to a successful CHD therapy [2].

MSCs have potential in promoting tissue repair and regeneration in adulthood, largely resulting from their multipotent and stem cell properties potentials [22]. The use of MSCs in recovering or regenerating injured hearts have some successes in pre-clinical researches, but the relatively big difference in the therapeutic outcome among various studies prevents its translation into clinical trials. Of note, this potent variation may result from the use of different preparation of MSCs in different studies, taking into account that MSCs are not a group of identical cells while different MSC subtypes may be prone to different cell lineage differentiation in future [19].




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