Date Published: February 15, 2019
Publisher: Impact Journals
Author(s): Zhiguo Zhao, Dan Gao, Tie Ma, Liping Zhang.
MicroRNAs (miRNAs) serve as regulatory factors in both healthy tissue and various cancers. Here, we used an miRNA microarray to screen for miRNAs differentially expressed between HNSCC and adjacent epithelial tissue. Among these, levels of miR-141 were significantly reduced in HNSCC tissues. Expression levels of epidermal growth factor receptor (EGFR) were enhanced in tissues with low miR-141 expression but were reduced by miR-141 overexpression, and there was a significant negative correlation between EGFR and miR-141 levels in HNSCC tissues (P < 0.01). Luciferase assays confirmed that miR-141 targeted EGFR mRNA. In vitro, miR-141 inhibited the proliferation and migration of Cal-27 and FaDu HNSCC cells with corresponding decreases in CDK4 and MMP2. miR-141 also enhanced the incidence of apoptosis among the cells with a corresponding decrease in bcl-2. In BALB/c mice injected with FaDu HNSCC cells, miR-141 mitigated hepatic metastasis and inhibited expression of EGFR, CDK4, bcl-2 and MMP2. These results suggest that miR-141 functions as a tumor suppressor in HNSCC and that it suppresses tumor growth and metastasis by suppressing EGFR signaling. MiR-141 thus appears to be a potentially useful therapeutic target in the treatment of HNSCC.
Head and neck squamous cell carcinoma (HNSCC) is the sixth most common type of human cancer, with more than 70% of patients suffering with recurrent or metastatic disease . There is thus an urgent need for effective therapeutic regimens to treat HNSCC.
In many tumors, levels of miR-141 expression are lower than in healthy tissue, and its increased expression can inhibit tumor progression . For example, miR-141 has been shown to inhibit the development of gastric, prostate, colorectal and liver cancers by inhibiting cell proliferation and metastasis and promoting cell apoptosis [12–14]. In our study, we found that miR-141 was weakly expressed in HNSCC tissues and that higher expression of miR-141 improves patients’ survival time.
MiR-141 functions as a tumor suppressor in HNSCC and that it suppresses tumor growth and metastasis by suppressing EGFR signaling.