Research Article: Muscle changes with high-intensity aerobic training in an animal model of renal disease1

Date Published: June 03, 2019

Publisher: Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia

Author(s): Eliane Barbosa Togoe, Iandara Schettert Silva, Juliana Loprete Cury, Flavia Alessandra Guarnier.


To analyze the muscle changes with high-intensity aerobic training (HIAT) in an animal model of renal disease (RD).

Twenty one adult Wistar rats were divided into 3 groups: healthy sedentary (HS), RD sedentary (RDS), RD aerobic training (RDAT). RDS and RDAT were subjected to unilateral renal ischemia-reperfusion (10 min) and 21days after that, RDAT was subjected to 6 weeks HIAT (swimming). Serum creatinine (Cr) and muscle morphometry (cross-sectional area = CSA) of gastrocnemius were analyzed.

Cr was higher (p = 0.0053) in RDS (0.82 ± 0.04) than in the others (RDAT 0.55 ± 0.04; HS 0.55 ± 0.04). Morphometric analysis (class interval of CSA in μm2/absolute frequency of muscle fibers in each class) indicated that 50th percentile occurred in: HS 7th class (3000.00-3499.00/515), RDS, 8th class (3500.00-3999.00/484), RDAT 5th class (2000.00-2499.00/856). CSA of largest fibers in RDS, RDAT, HS was 9953.00 μm2, 9969.00 μm2,11228.00 μm2, respectively. High frequency of fibers with lower CSA occurred in 4th, 5th, 6th and 7th class in RDA, absence of fibers into 22nd, 23rd classes (RDS and RDAT).

HIAT in an animal model of RD resulted in increased the number of muscle fibers with smaller CSA.

Partial Text

Chronic kidney disease (CKD) is a remarkable public health concern associated with an increased risk of mortality and comorbidities. It is estimated that 10%-15% of the general population is affected by CKD worldwide1.

This study was approved by the Animal Research Ethics Committee, Universidade Federal do Mato Grosso (UFMS) under Protocol No. 735/2015.

The results of comparison of body weight, maximum load in incremental tests, and serum creatinine levels in the study groups are shown in Table 1.

Aerobic exercise has been shown to have several benefits, including improvement in physical capacity, cardiovascular function, and metabolic regulation8. In this study, the effect of HIAT on muscle tissue was assessed in an animal model of RD.

The results demonstrated that HIAT in an animal model of RD increased the number of muscle fibers of smaller CSA.




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