Date Published: November 30, 2015
Publisher: Public Library of Science
Author(s): Fenqin Chen, Ning Zhang, Xiaoyu Ma, Ting Huang, Ying Shao, Can Wu, Qiuyue Wang, Utpal Sen.
Naringin, a flavanone glycoside extracted from Citrus grandis Osbeck, has a wide range of pharmacological effects. In the present study we aimed at demonstrating the protective effect of naringin against diabetic kidney disease (DKD) and elucidating its possible molecular mechanism underlying. The beneficial effect of naringin was assessed in rats with streptozotocin (STZ)-induced diabetes and high glucose-induced HBZY-1 cells. According to our results, first we found that naringin relieved kidney injury, improved renal function and inhibited collagen formation and renal interstitial fibrosis. Second, we confirmed that naringin restrained oxidative stress by activating Nrf2 antioxidant pathway. Moreover, the results suggested that naringin significantly resisted inflammatory reaction by inhibiting NF- κ B signaling pathway. Taken together, our results demonstrate that naringin effectively alleviates DKD, which provide theoretical basis for naringin clinically used to treatment of DKD.
Diabetes is a metabolic disorder syndrome characterized by a chronically raised blood glucose level, which is caused by various kinds of factors including hereditary factor, immune dysfunction, microbial infection, energetic factor and so on. Diabetes as a systemic metabolic disease, has a number of chronic or acute complications, such as diabetic kidney disease (DKD), retinopathy, hypertension, diabetic food and diabetic ketoacidosis. DKD is the most common and severe chronic complication of diabetes and is also the major microvascular complication, which is morphologically characterized by excessive accumulation of the extracellular matrix and could ultimately progress to tubulointerstitial fibrosis[3, 4]错误!超链接引用无效。错误!超链接引用无效。. There is no symptoms of the early diabetes, and with the course of illness the incidence rate of DKD is increased. DKD is one of the main causes of death in diabetic patients. However, at present there are few effective treatment options for DKD, so the development of new therapeutic approaches will be of great importance in alleviating DKD and reducing the mortality rate of diabetic patients.
Given DKD does great harm to human, selecting and identifying effective drugs on prevention and treatment of DKD has been the hot research. In this study, we focused on investigating the protective effects of naringin, a bioactive glucoside of pomelo (Citrus grandis Osbeck) widely applied to food, pharmaceutical and cosmetic, and elucidated the potential molecular mechanisms. The highest dose of naringin in this study is roughly equivalent to 100 g citrus fruits, which is close to normal daily human intake. Mullen et al. reported that the glucosides can be existed by glucosyltransferase in kidney . So the research of naringin against kidney injury induced by diabetics has strong realistic meaning. Our study finds some new interesting information about naringin treatment alleviating DKD via in vivo and in vitro studies. This is the first time that naringin has been reported to have protective effect on DKD.