Research Article: Olive oil bioactives protect pigs against experimentally-induced chronic inflammation independently of alterations in gut microbiota

Date Published: March 27, 2017

Publisher: Public Library of Science

Author(s): Martin Liehr, Alessandro Mereu, Jose Javier Pastor, Jose Carlos Quintela, Stefanie Staats, Gerald Rimbach, Ignacio Rodolfo Ipharraguerre, Hauke Smidt.

http://doi.org/10.1371/journal.pone.0174239

Abstract

Subclinical chronic inflammation (SCI) is associated with impaired animal growth. Previous work has demonstrated that olive-derived plant bioactives exhibit anti-inflammatory properties that could possibly counteract the growth-depressing effects of SCI. To test this hypothesis and define the underlying mechanism, we conducted a 30-day study in which piglets fed an olive-oil bioactive extract (OBE) and their control counterparts (C+) were injected repeatedly during the last 10 days of the study with increasing doses of Escherichia coli lipopolysaccharides (LPS) to induce SCI. A third group of piglets remained untreated throughout the study and served as a negative control (C-). In C+ pigs, SCI increased the circulating concentration of interleukin 1 beta (p < 0.001) and decreased feed ingestion (p < 0.05) and weight gain (p < 0.05). These responses were not observed in OBE animals. Although intestinal inflammation and colonic microbial ecology was not altered by treatments, OBE enhanced ileal mRNA abundance of tight and adherens junctional proteins (p < 0.05) and plasma recovery of mannitol (p < 0.05) compared with C+ and C-. In line with these findings, OBE improved transepithelial electrical resistance (p < 0.01) in TNF-α-challenged Caco-2/TC-7 cells, and repressed the production of inflammatory cytokines (p < 0.05) in LPS-stimulated macrophages. In summary, this work demonstrates that OBE attenuates the suppressing effect of SCI on animal growth through a mechanism that appears to involve improvements in intestinal integrity unrelated to alterations in gut microbial ecology and function.

Partial Text

Inflammation is a protective mechanism of higher organisms that aids in coping with stressors and harmful environmental stimuli [1]. Despite being tremendously complex and involving a variety of immune cells, blood vessels, and molecular mediators, inflammatory processes can be distinguished into two somewhat different types [2; 3] termed herein clinical and subclinical chronic inflammation (SCI). In contrast to clinical inflammation resulting for instance from injury or bacterial infection, SCI appears as a much milder but persistent response of the host’s immune system [4].

Our data indicate that dietary supplementation with OBE can alleviate detrimental effects of SCI by modulating the immune-inflammatory response of pigs and thereby inducing persistent effects on animal health and performance.

Overall, data reported herein demonstrate that olive-derived bioactive compounds have growth-permitting action in pigs challenged experimentally with SCI. The underlying mode of action apparently includes anti-inflammatory effects, in particular inhibition of IL1B production and protection of intestinal integrity unrelated to alterations in gut microbial ecology (Fig 7). Taken together, supplementing the diet of pigs with OBE might represent a promising strategy to counteract SCI-related disorders in commercial settings of pig production.

 

Source:

http://doi.org/10.1371/journal.pone.0174239

 

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