Research Article: Oxidative stress assessment in intestine of newborn rats submitted to hypoxia and reoxygenation with tadalafil1

Date Published: April 29, 2019

Publisher: Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia

Author(s): Luciana Cristina Teixeira, Renato Toshio Murashita Fujiki, Caio Vinicius Coitinho Paiva de Souza, Camila Mendes de Souza, Maysa Moreira Mismetti, Ricardo Artigiane-Neto, Edna Frasson de Souza Montero, José Luiz Martins.


To evaluate the functional and structural response of tadalafil effects in the intestinal mucosa, using an experimental model of hypoxia and reoxygenation injury in rats.

The animals were divided into 4 groups: CTL, H/R, H/R+Td and M+Td. The newborn rats allocated in groups H/R, H/R+Td and M+Td were submitted twice a day, to a gas chamber with CO2 at 100% for 10 minutes and afterward reoxygenation with O2 at 98% for 10 minutes, in the three first days of life. Tadalafil dose was given to newborn of group H/R+Td and to the pregnant rat of group M+Td. Histological analysis was made with hematoxylin-eosin technique and oxidative stress through nitrite and nitrate levels and lipid peroxidation.

The histological analysis showed a reduction of mucosa alterations in the groups that received tadalafil. In the oxidative stress evaluation, occurred an increase of NO levels and less lipidic peroxidation in the ileum segments that received tadalafil.

Tadalafil provides tissue protection when administered independently to both, pregnant or newborns.

Partial Text

The necrotizing enterocolitis (NEC) is one of the most common gastrointestinal emergencies in newborn1, its main risk factor is the prematurity, which associated to formula feeding, leading to bacterial invasion and triggering intestinal ischemia/ reperfusion phenomena (H/R)2. It is estimated that the disease affects between 5% and 15% of premature and about 7% of term infants admitted into neonatal intensive care units and that the mortality rate ranges between 18% and 45%, depending on the prematurity degree1.

All procedures performed in the current study were evaluated and approved by the Ethics Committee for Animal Use – UNIFESP, under number 2739041117.

Based on the results obtained tadalafil could be a promising drug in the treatment and prevention of H/R damage, especially when provided during the prenatal period.

Tadalafil provides tissue protection, independent of those given for pregnant or in newborns.




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