Research Article: Poly (Lactic Acid) membrane and Sedum dendroideum extract favors the repair of burns in rats1

Date Published: May 11, 2020

Publisher: Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia

Author(s): Juliane Peliçari Binotto, Larissa Giorgetti Mendes, Fernanda Oliveira de Gaspari Gaspi, Marcelo Augusto Marreto Esquisatto, Thiago Antonio Moretti de Andrade, Fernanda Aparecida Sampaio Mendonça, Gláucia Maria Tech Santos.

http://doi.org/10.1590/s0102-865020200030000002

Abstract

To evaluate the healing potential of the electrospinning membranes of Poly (Lactic Acid) (PLA) associated with Sedum dendroideum extract in burn injuries in rats.

Seventy-five rats were submitted to burn injury on their back skin: (C) untreated; (F) with daily topical application of S. dendroideum extract; (M) with electrospinning membranes of PLA; (MF10) with electrospinning membranes of PLA with 10% S. dendroideum extract; (MF25) with electrospinning membranes of PLA with 25% S. dendroideum extract. Tissue samples were taken after 2, 6 and 14 days of the burn injury and were subjected to histomorfometric analysis of quantification of fibroblasts, collagen fibers, blood vessels, and inflammatory infiltrate

The histomorphometric analysis showed an increase in the number of fibroblasts, collagen fibers and blood vessels in the burns treated with membranes of PLA, associated or not with the 10% and 25% extract. The extract of S. dendroideum promoted the increase of collagen fibers.

The electrospinning PLA membrane, isolated or associated with the S. dendrodeum extract, favored the healing of burn injuries in this experimental model, with an increase of fibroblasts, collagen fibers, and blood vessels. S. dendroideum isolated only stimulated the collagenesis.

Partial Text

Burns of II-IIIa degree makes the majority of thermal injuries when treatment is mainly carried out by conservative methods. The problem of the covering of large burned surfaces is still a challenge1,2. The treatment of burn includes removal of dead tissues (debridement), dressing of the wound, fluid recovery, antibiotics administration, skin grafting (autografts, allografts, and xenografts), cultured dermal substitute and transplantation of keratinocytes, besides dressings like Silver Sulfadiazine and specific herbals3. A wide variety of preparations and remedies of non-organic, organic, biogenic, and phytogenic origin have been devised and used in the topical treatment of burns4.

The membranes obtained for this study did not present the expected morphology for the electrospinng technique, as can be observed in the SEM images (Fig. 1). The addition of S. dendroideum extract modified the properties of the solution, causing the formation of beads interconnected by nanofibers, in the membrane structure. However, the whole structure obtained showed a high degree of porosity, which is an excellent feature for use as a scaffold, since there is an increase of the contact surface for cell growth in the healing process.

Given the difficulties in establishing a noninvasive therapy for the treatment of burns, new techniques are sought to promote rapid and effective occlusion for this type of injury, especially if the occlusives are healing1. Different polymers capable of interacting with cells have been developed to promote tissue growth and differentiation8. Among these the Poly (Lactic Acid) (PLA) stands out for promoting prolonged action in the tissue23.

The PLA electrospinning membrane, isolated or associated with S. dendroideum extract, promoted angiogenesis, fibroplasias, and collagenesis in the process of tissue repair of second-degree burns on the back skin of rats. In turn, S. dendroideum isolated only stimulated the collagenesis. However, for indicating the biomaterial in the repair of difficult healing wounds, it is important to have continuity of studies using scaffolds and herbal medicines for better safety in therapy. The molecular analysis also should be performed in future researches to better understand the findings so far, and its absence is considered a limitation in this study.

 

Source:

http://doi.org/10.1590/s0102-865020200030000002

 

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