Research Article: Proteomic analysis of rat serum revealed the effects of chronic sleep deprivation on metabolic, cardiovascular and nervous system

Date Published: September 20, 2018

Publisher: Public Library of Science

Author(s): Bo Ma, Jincheng Chen, Yongying Mu, Bingjie Xue, Aimei Zhao, Daoping Wang, Dennis Chang, Yinghong Pan, Jianxun Liu, Jon M. Jacobs.

http://doi.org/10.1371/journal.pone.0199237

Abstract

Sleep is an essential and fundamental physiological process that plays crucial roles in the balance of psychological and physical health. Sleep disorder may lead to adverse health outcomes. The effects of sleep deprivation were extensively studied, but its mechanism is still not fully understood. The present study aimed to identify the alterations of serum proteins associated with chronic sleep deprivation, and to seek for potential biomarkers of sleep disorder mediated diseases. A label-free quantitative proteomics technology was used to survey the global changes of serum proteins between normal rats and chronic sleep deprivation rats. A total of 309 proteins were detected in the serum samples and among them, 117 proteins showed more than 1.8-folds abundance alterations between the two groups. Functional enrichment and network analyses of the differential proteins revealed a close relationship between chronic sleep deprivation and several biological processes including energy metabolism, cardiovascular function and nervous function. And four proteins including pyruvate kinase M1, clusterin, kininogen1 and profilin-1were identified as potential biomarkers for chronic sleep deprivation. The four candidates were validated via parallel reaction monitoring (PRM) based targeted proteomics. In addition, protein expression alteration of the four proteins was confirmed in myocardium and brain of rat model. In summary, the comprehensive proteomic study revealed the biological impacts of chronic sleep deprivation and discovered several potential biomarkers. This study provides further insight into the pathological and molecular mechanisms underlying sleep disorders at protein level.

Partial Text

Sleep is an essential and fundamental physiological process that plays crucial roles in the balance of psychological and physical health in almost all animals [1]. Accumulating evidence has demonstrated that sleep and wakefulness are critical to the cellular homeostasis associated with energy metabolism, synaptic potentiation, and responses to cellular stress [2]. In modern time, an increasing number of people have insufficient sleep and suffer from chronic sleep deprivation (CSD) [3, 4].

In the present study, we used a label-free quantitative proteomics technology to survey the global changes in serum protein abundance between the CSD rats and the normal rats to further elucidate the mechanisms underlying sleep disorder and relating diseases. Comparison analysis revealed a significant quantitative alteration in protein with differential abundance between the two groups. Bioinformatics and functional analyses showed a strong link between CSD and energy metabolism, cardiovascular function and nervous system. In addition, four protein candidates including PKM, CLU, KNG1 and PFN1 were identified as potential biomarkers for sleep disorder related diseases.

 

Source:

http://doi.org/10.1371/journal.pone.0199237

 

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