Research Article: Quality and quantity of dromedary camel DNA sampled from whole-blood, saliva, and tail-hair

Date Published: January 31, 2019

Publisher: Public Library of Science

Author(s): Hasan Alhaddad, Tasneem Maraqa, Suha Alabdulghafour, Huda Alaskar, Randa Alaqeely, Faisal Almathen, Bader H. Alhajeri, Arnar Palsson.

http://doi.org/10.1371/journal.pone.0211743

Abstract

Camels are livestock with unique adaptations to hot-arid regions. To effectively study camel traits, a biobank of camel DNA specimens with associated biological information is needed. We examined whole-blood, saliva (buccal swabs), and tail-hair follicle samples to determine which is the best source for establishing a DNA biobank. We inspected five amounts of each of whole-blood, buccal swabs, and tail-hair follicles in nine camels, both qualitatively via gel electrophoresis and quantitatively using a NanoDrop spectrophotometer. We also tested the effects of long term-storage on the quality and quantity of DNA, and measured the rate of degradation, by analyzing three buccal swab samples and 30 tail-hair follicles over a period of nine months. Good quality DNA, in the form of visible large size DNA bands, was extracted from all three sources, for all five amounts. The five volumes of whole-blood samples (20–100μl) provided ~0.4–3.6 μg, the five quantities of buccal swabs (1–5) produced ~0.1–12 μg, while the five amounts of tail-hair follicles (10–50) resulted in ~0.7–25 μg. No differences in the rate of degradation of buccal swab and tail-hair follicle DNA were detected, but there was clearly greater deterioration in the quality of DNA extracted from buccal swabs when compared to tail-hair follicles. We recommend using tail-hair samples for camel DNA biobanking, because it resulted in both an adequate quality and quantity of DNA, along with its ease of collection, transportation, and storage. Compared to its success in studies of other domesticated animals, we anticipate that using ~50 tail-hair follicles will provide sufficient DNA for sequencing or SNP genotyping.

Partial Text

Dromedary camels (Camelus dromedarius Linnaeus, 1758) are multi-purpose livestock, domesticated for their adaptations to survive, reproduce, and produce (e.g. milk and meat) in hot-arid environments [1]. The two main hot-desert adaptations in dromedary camels are both extreme thermal tolerance and their high level of water conservation [2].

In this study, we focused on camel DNA sources most suitable for establishing a DNA biobank. Whole-blood, saliva (buccal swabs), and tail-hair follicles were examined for ease of collection, storage, and transportation. A comparison of the quality and quantity of DNA extracted from each of these three sources was compared, with specific emphasis on their potential utility for subsequent genetic analyses (e.g. quantity, degradation tolerance).

 

Source:

http://doi.org/10.1371/journal.pone.0211743

 

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