Research Article: Relative expression of TAp73 and ΔNp73 isoforms

Date Published: March 4, 2012

Publisher: Impact Journals LLC

Author(s): Franco Conforti, Ai Li Yang, Massimiliano Agostini, Alessandro Rufini, Paola Tucci, Maria Victoria Nicklison-Chirou, Francesca Grespi, Tania Velletri, Richard A. Knight, Gerry Melino, Berna S. Sayan.

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Abstract

The transcription factor p73 belongs to the p53 family of tumour suppressors and similar to other family members, transcribed as different isoforms with opposing pro- and anti-apoptotic functions. Unlike p53, p73 mutations are extremely rare in cancers. Instead, the pro-apoptotic activities of transcriptionally active p73 isoforms are commonly inhibited by over-expression of the dominant negative p73 isoforms. Therefore the relative ratio of different p73 isoforms is critical for the cellular response to a chemotherapeutic agent. Here, we analysed the expression of N-terminal p73 isoforms in cell lines and mouse tissues. Our data showed that the transcriptionally competent TAp73 isoform is abundantly expressed in cancer cell lines compared to the dominant negative ΔNp73 isoform. Interestingly, we detected higher levels of ΔNp73 in some mouse tissues, suggesting that ΔNp73 may have a physiological role in these tissues.

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