Research Article: Stimulating growth and xylindein production of Chlorociboria aeruginascens in agar-based systems

Date Published: March 12, 2012

Publisher: Springer

Author(s): Sara C Robinson, Daniela Tudor, Hilary Snider, Paul A Cooper.


Four isolates of Chlorociboria aeruginascens were tested for possible stimulatory effects when grown on malt agar media containing wood additives. The addition of any of the four types of test wood (Acer saccharum, Populus tremuloides, spalted P. tremuloides, and Ailanthus altissima), stimulated colony growth and xylindein production in C. aeruginascens. Addition of any amount of wood produced more growth than no wood additions, while ground wood produced more growth than chopped wood. Of the wood types tested, A. saccharum wood stimulated all four isolates, while spalted Populus tremuloides stimulated three of the four isolates. High glucose and sucrose amounts may be partially responsible for the greater stimulatory affect of some woods over others. The development of this simple and reliable method for growth and pigment stimulation of C. aeruginascens in laboratory conditions will allow for further development of this fungus for decorative and commercial use.

Partial Text

The Chlorociboria genus is widely distributed throughout the world; Chlorociboria aeruginascens (Nyl.) Kanouse and Chlorociboria aeruginosa (Oeder) Seaver are native to North America (Ramamurthi et al. 1957). Xylindein, the penetrating blue-green pigment produced by C. aeruginascens, (Mizuki et al. 2003), can be readily found in forests on decaying wood, especially Populus sp. (Blanchette et al. 1992) and Quercus sp. (Dennis 1956).

Chlorociboria aeruginascens SR003, originally isolated from a decaying Populus sp. log in Keweenaw County, MI was grown on plates containing varying amounts and sizes of ground wood mixed into agar media. Following inoculation, the diameter of each colony was recorded.

The addition of wood particles to agar media is used occasionally for mycological work, although its addition to growth media can affect fungi in very different ways. The addition of wood to agar induces the production of perithecium and ascospores of Ceratocystis pilifera (Fr.) (Zimmerman et al. 1995) and increases the growth rate of certain blue staining fungi (Shrimpton & Whitney 1968). However, wood based agar is also occasionally utilized as a way to limit growth on media (Lonergan et al. 1993). It is apparent from this study that the addition of any wood to media plates inoculated with C. aeruginascens does not retard growth or xylindein production, and in many cases appears to stimulate both.

The authors declare that they have no competing interests.