Research Article: The effect of Allium sativum in experimental peritoneal adhesion model in rats1

Date Published: December 09, 2019

Publisher: Sociedade Brasileira para o Desenvolvimento da Pesquisa em

Author(s): Uğur Topal, Nuri Emrah Göret, Ceren Canbey Göret, Ömer Faruk Özkan.


To evaluate the effect of garlic on formation of postoperative adhesions in

Twenty-four Sprague dawley rats were divided into three groups. In Group 1
(sham), laparotomy was performed and stitched up. In Group 2 (control),
after laparotomy was performed, punctate hemorrhage was induced by cecal
abrasion in the cecum and 2 cc of saline was intraperitoneally administered
to each rat. In Group 3 (experimental), after laparotomy was performed,
punctate hemorrhage was induced by cecal abrasion in the cecum and each rat
was intraperitoneally administered a sterile Allium sativum derivative. The
rats in all groups were re-laparotomized on postoperative day 7; samples
were obtained from the peritoneal tissue surrounding the cecum

In Group 3, there was a statistically significant difference in terms of
inflammation, lymph node size, and free oxygen radicals; these parameters
tended to increase. In terms of fibrosis evaluated using H&E and MT,
there was no significant difference between groups 2 and 3.

No positive outcomes indicating that Allium sativum reduces intra-abdominal
adhesions were obtained. However, it caused severe inflammation in the
tissue. Additionally, in immunohistochemical analyses conducted to detect
oxidative stress, allium sativum increased the production of free oxygen
radicals in the tissue.

Partial Text

Postoperative peritoneal adhesions (PA), defined as fibrous band formation between
intra-abdominal organs, is one of the most important problems of surgery. It is
stated that the frequency of PA increases up to 90% in some sources1. Abdominal reoperation is more difficult and riskier when there are
adhesions. Increased enterotomy risk, longer dissection times and longer operation
times are serious consequences of intraabdominal adhesions2,3.

From statistical analyses, the results of specimens in Groups 1, 2, and 3 were not
normally distributed; therefore, they were compared using the Kruskal-Wallis

Peritoneal adhesions (PA) remain a major health problem and, according to some
sources, have been reported to be seen at very high rates, especially after
abdominal surgery1. Intestinal obstruction, abdominal pain due to visceral peritoneal traction,
infertility due to extraluminal compression, urinary dysfunction due to traction of
ureters may be seen secondary to PA13.

In this study, no positive results were found that Allium sativum had positive
effects on fibrosis, inflammation or reactive lymph node size/number which are among
the intraabdominal adhesion parameters; in addition, immunohistochemical analysis to
detect oxidative stress at the cellular level did not show a positive decrease in
free oxygen radicals.




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