Date Published: April , 2009
Publisher: A.I. Gordeyev
Author(s): K. A. Konduktorov, G. S. Lyudva, A. V. Ivanov, V. L. Tunitskaya, S. N. Kochetkov.
Hepatitis C is one of the most dangerous and widespread viral diseases. Currently, the World Health Organization estimates that about 170 million people are infected with the hepatitis C virus (HCV), the causative agent of infection, in almost every country in the world. The RNA-dependent RNA-polymerase (R-RNAP, virus nonstructural protein) is a key fragment that carries out HCV genome replication. R-RNAP is about 65 kDA in molecular weight and localized on the endoplasmic reticulum membrane of infected hepatic cells by the C-tail α-spiral transmembrane domain (21 a.r.). One characteristic feature of R-RNAP is its ability to catalyze RNA synthesis by both primer-dependent and primer-independent (de novo) mechanisms . In the first case, in the in vitro experiments, the primer-matrix poly(rA)-oligo(rU) duplex is used as the RNA matrix; in the second case, the HCV genome fragments are used. It is suggested that oligomer from several identical R-RNAP molecules takes part in the replication. Moreover, oligomer was discovered to be composed of H502 and E18 amino-acid residues located in the interaction area of protein globules .