Research Article: Use of thermography in skin grafts after the application of therapeutic ultrasound in Wistar rats1

Date Published: August 05, 2020

Publisher: Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia

Author(s): Ynaiê Garcia da Silveira, Caroline Kajiura, Filippo Jannoni Lessa Bernardes, Sônia Prince Maria, Marina Pugnaghi Fernandes, Marla Tereza Frasson, Pedro Cassino, Stella Habib Moreira, Jorge Luis Álvarez Gómez, Josiane Morais Pazzini, Andrigo Barboza de Nardi, Paola Castro Moraes.

http://doi.org/10.1590/s0102-865020200070000003

Abstract

To analyze changes in the thermal pattern in the skin graft receptor bed, after the use of therapeutic ultrasound through the thermographic images.

Eighteen Rattus norvegicus albinus Wistar, separated into two groups: GST groups (without tumor and without treatment with ultrasound) and GT (with tumor and treatment with ultrasound). In the GT group, induction of carcinogenesis was performed by single intradermal application of 0.05 ml DMBA at 0.5%, diluted in acetone. Subsequently, a technique of reconstructive grafting surgery of the mesh type was performed in both groups and treatment with therapeutic ultrasound was performed in the GT group the alternate day protocol at 3, 6, 10 and 15 days after the procedure. The thermographic evaluation occurred on days 3, 6, 10 and 15 after the grafting.

There was a significant difference between the statistical evaluation of the temperature of the control group when compared to the treated group, on the different evaluation days (p <0.0001). The thermographic analysis of the images was effective in evaluating the healing process, being the use of thermography feasible to evaluate changes in the thermal standard in the surgical bed, besides the beneficial effects of the US.

Partial Text

Assuming the physical consideration that every living organism produces heat and thereby emits infrared radiation directly proportional to its temperature, thermography consists of the immediate and non-aggressive evaluation of the mapping of this radiation charge, expressing the gradual gradient variation in color pattern in a thermogram1. According to Corte and Hernandez2, when detecting infrared radiation, innumerable alterations related to changes in blood flow can be identified, being possible the recognition of vascular, neurological and muscular functional alterations.

This study was approved by the Committee on Ethics in the Use of Animals (CEUA), Universidade Estadual Paulista (UNESP), campus Jaboticabal (Protocol number 00997917).

There was a significant difference between the control group when compared to the treated group on the different evaluation days. In the treated group, there was a significant difference on days 5, 10 and 15 (p <0.0001), and it was possible to observe that the temperature raised on the fifth day and remained stable until the end of the evaluations. In the control group, the temperature decreased at the beginning of the healing phase (p <0.0001), between days 5 and 10, to recover on day 15, whereas in the GT group it was significantly higher (p <0.0001) on days 5, 10 and 15 than on day 3 (Table 1). However, the average temperature, minimum and maximum, showed the same behavior (p = 0.7890) when compared between groups. According to Oliveira et al.17, the effect of temperature on healing is directly related to its effect on peripheral vasomotor tone, exerting autonomic reflex vasodilation, which promotes increased local vasodilation and oxygenation and tissue nutrition, indicating the formation of new vessels during the process scarring. Therefore, it can be inferred that healing was favorable in the animals treated with therapeutic ultrasound, since the increase of the temperature promotes the expansion of new vessels, denominated angiogenesis, not being observed in the control group that did not receive therapeutic ultrasound. The thermographic analysis of the images was effective in evaluating the healing process, being the use of thermography feasible to evaluate changes in the thermal standard in the surgical bed, besides the beneficial effects of the US.   Source: http://doi.org/10.1590/s0102-865020200070000003

 

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